GDI1 Human

GDP Dissociation Inhibitor 1 Human Recombinant
Cat. No.
BT10524
Source
Escherichia Coli.
Synonyms
Rab GDP dissociation inhibitor alpha, Rab GDI alpha, GDP Dissociation Inhibitor 1, GDI1, Guanosine diphosphate dissociation inhibitor 1, GDI-1, Oligophrenin-2, XAP-4, GDIL, OPHN2, RABGDIA, XAP4, MRX41, MRX48, 1A, RABGD1A.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

GDI1 Human Recombinant produced in E. coli is a single polypeptide chain containing 470 amino acids (1-447) and having a molecular mass of 53 kDa.
GDI1 is fused to 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
GDP Dissociation Inhibitor 1 (GDI1) is primarily found in nerve and sensory tissues. It plays a crucial role in regulating the activity of Rab proteins, which are involved in intracellular transport. GDI1 prevents the activation of Rab proteins by inhibiting the exchange of GDP for GTP and promoting their association with membranes. Mutations in the GDI1 gene have been linked to X-linked nonspecific mental retardation.
Description
Recombinant human GDI1 protein was produced in E. coli and consists of 470 amino acids (residues 1-447) with a molecular weight of 53 kDa. It includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
A clear, sterile solution.
Formulation
The GDI1 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freezing and thawing.
Purity
Purity is greater than 90% as assessed by SDS-PAGE analysis.
Synonyms
Rab GDP dissociation inhibitor alpha, Rab GDI alpha, GDP Dissociation Inhibitor 1, GDI1, Guanosine diphosphate dissociation inhibitor 1, GDI-1, Oligophrenin-2, XAP-4, GDIL, OPHN2, RABGDIA, XAP4, MRX41, MRX48, 1A, RABGD1A.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDEEYDV IVLGTGLTEC ILSGIMSVNG KKVLHMDRNP YYGGESSSIT PLEELYKRFQ LLEGPPESMG RGRDWNVDLI PKFLMANGQL VKMLLYTEVT RYLDFKVVEG SFVYKGGKIY KVPSTETEAL ASNLMGMFEK RRFRKFLVFV ANFDENDPKT FEGVDPQTTS MRDVYRKFDL GQDVIDFTGH ALALYRTDDY LDQPCLETVN RIKLYSESLA RYGKSPYLYP LYGLGELPQG FARLSAIYGG TYMLNKPVDD IIMENGKVVG VKSEGEVARC KQLICDPSYI PDRVRKAGQV IRIICILSHP IKNTNDANSC QIIIPQNQVN RKSDIYVCMI SYAHNVAAQG KYIAIASTTV ETTDPEKEVE PALELLEPID QKFVAISDLY EPIDDGCESQ VFCSCSYDAT THFETTCNDI KDIYKRMAGT AFDFENMKRK QNDVFGEAEQ.

Product Science Overview

Function and Mechanism

GDI1 functions by inhibiting the dissociation of GDP from Rab proteins and subsequently preventing the binding of GTP to them. This regulation is essential for maintaining the proper function and localization of Rab proteins within the cell. Specifically, GDI1 promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation .

Expression and Localization

GDI1 is primarily expressed in neural and sensory tissues. It is found in various cellular components, including the cytoplasm, cytosol, Golgi apparatus, myelin sheath, and neuron projections . The protein is also involved in several biological processes such as Rab protein signal transduction, regulation of small GTPase mediated signal transduction, and vesicle-mediated transport .

Clinical Significance

Mutations in the GDI1 gene have been linked to X-linked nonspecific mental retardation and intellectual developmental disorders . These mutations can disrupt the normal function of GDI1, leading to various neurological and cognitive impairments.

Recombinant GDI1

Recombinant GDI1 refers to the protein produced through recombinant DNA technology, which involves inserting the GDI1 gene into a suitable expression system to produce the protein in large quantities. This recombinant protein is used in various research and clinical applications to study its function and role in cellular processes.

Research and Applications

Research on GDI1 has provided significant insights into its role in cellular trafficking and its impact on neurological disorders. The recombinant form of GDI1 is particularly valuable in studying the protein’s structure, function, and interactions with other cellular components. It also serves as a tool for developing potential therapeutic strategies for disorders associated with GDI1 dysfunction.

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