GDF7 Mouse

GDF-7 was first identified as a protein involved in the development and differentiation of various tissues. It is synthesized as a large precursor protein that consists of an N-terminal signal sequence, a pro-region, and a C-terminal mature peptide . The mature peptide is responsible for the biological activity of GDF-7.

GDF-7 plays a crucial role in the development and repair of tissues. It is particularly important in the differentiation of tendon and ligament cells, as well as in the repair of injured organs . GDF-7 has been shown to promote the expansion of hepatic progenitor cells in liver fibrosis, suggesting a potential pro-regenerative function .

GDF-7 exerts its effects by binding to specific receptors on the surface of target cells. These receptors are part of the BMP receptor family, which includes BMPR1A and BMPR2 . Upon binding to these receptors, GDF-7 activates intracellular signaling pathways that lead to the expression of genes involved in cell growth, differentiation, and repair.

Recombinant Mouse GDF-7 is produced using E. coli expression systems. The recombinant protein is purified to high levels of purity and is available in both carrier-free and carrier-containing formulations . The carrier protein, typically Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .

Recombinant Mouse GDF-7 is used in various research applications, including:

• Cell and tissue culture: To study the effects of GDF-7 on cell differentiation and tissue repair.
• ELISA standards: As a standard in enzyme-linked immunosorbent assays (ELISAs) to quantify GDF-7 levels in biological samples.
• In vitro studies: To investigate the signaling pathways activated by GDF-7 and its role in tissue development and repair .

Recombinant Mouse GDF-7 is typically stored at -20 to -70°C to maintain its stability. It is important to avoid repeated freeze-thaw cycles to prevent degradation of the protein .