GDF7 Human
Growth and Differentiation factor 7 Human Recombinant
Cat. No.
BT18552
Source
Escherichia Coli.
Synonyms
Growth Differentiation Factor 7, GDF-7, Growth/Differentiation Factor 7, BMP12, GDF7.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GDF7 Human Recombinant (322-450) produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 129 amino acids and having a molecular mass of 28kDa.
The GDF-7 is purified by proprietary chromatographic techniques.
The GDF-7 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Growth Differentiation Factor-7 (GDF-7), a protein belonging to the Bone Morphogenetic Protein (BMP) family within the Transforming Growth Factor-beta (TGF-b) superfamily, exerts its biological effects by binding to a heterodimeric receptor complex. This complex consists of a type 1 receptor (BMPR-IB) and a type II receptor (BMPR-II or Activin RII), both of which are serine/threonine kinase receptors. Upon GDF-7 binding, Smad proteins are phosphorylated and activated, initiating downstream signaling. GDF-7 is implicated in various physiological processes, including the formation and repair of tendons and ligaments, regulation of bone formation, differentiation of mesenchymal stem cells, differentiation of neuronal cells, and guidance of axon growth.
Description
Recombinant human GDF7, spanning amino acids 322 to 450, is produced in E. coli. This protein is a disulfide-linked homodimer, lacking glycosylation, and comprises a polypeptide chain of 129 amino acids. It has a molecular weight of 28 kDa. The purification of GDF-7 is achieved through proprietary chromatographic techniques.
Physical Appearance
The product appears as a white powder, obtained through sterile filtration and lyophilization (freeze-drying).
Formulation
Prior to lyophilization, the product is in a 0.2µm filtered solution of hydrochloric acid (HCl).
Solubility
To reconstitute the lyophilized GDF-7, it is recommended to dissolve it in sterile 18M-cm H2O at a concentration of at least 100µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized GDF7 remains stable at room temperature for up to 3 weeks, but for long-term storage, it should be kept desiccated at a temperature below -18°C. After reconstitution, GDF-7 should be stored at 4°C for a period of 2 to 7 days. For extended storage, freezing at -18°C is recommended. To enhance stability during long-term storage, it is advisable to add a carrier protein, such as 0.1% HSA or BSA. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the product, as determined by SDS-PAGE analysis, is greater than 95.0%.
Biological Activity
The biological activity of GDF-7 is measured by its ability to induce alkaline phosphatase production in mouse ATDC5 cells. The ED50, which represents the concentration required for half-maximal induction, is less than 1.25µg/ml.
Synonyms
Growth Differentiation Factor 7, GDF-7, Growth/Differentiation Factor 7, BMP12, GDF7.
Source
Escherichia Coli.
Amino Acid Sequence
TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR.
Product Science Overview
Structure and Synthesis
Biological Functions
GDF7 plays a crucial role in several biological processes, including:
- Tendon and Ligament Formation: GDF7 is involved in the formation and repair of tendons and ligaments .
- Hepatic Progenitor Cell Expansion: Recent studies have shown that GDF7 promotes the expansion of hepatic progenitor cells in liver fibrosis, suggesting a potential pro-regenerative function .
- Chondrogenesis: GDF7 has been shown to induce alkaline phosphatase production in chondrogenic cells, which is a marker of cartilage formation .
Mechanism of Action
GDF7 exerts its effects through a heterodimeric receptor complex composed of:
- A type I serine/threonine kinase receptor (BMPR-IB)
- A type II serine/threonine kinase receptor (BMPR-II or Activin RII)
Upon binding to its receptors, GDF7 activates the Smad signaling pathway, leading to the phosphorylation and activation of Smad proteins . These activated Smad proteins then translocate to the nucleus, where they regulate the expression of target genes involved in cell growth and differentiation.
Clinical Implications
The role of GDF7 in tissue repair and regeneration has significant clinical implications. For instance:
- Liver Fibrosis: GDF7 has been shown to promote liver organoid growth and enhance the expression of progenitor cell markers in fibrotic liver tissue . This suggests that GDF7 could be utilized for the treatment of hepatic fibrosis caused by chronic liver diseases.
- Tendon and Ligament Injuries: Due to its role in tendon and ligament formation, GDF7 could be a potential therapeutic agent for the treatment of tendon and ligament injuries .
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