GCSF Rat
Granulocyte-Colony Stimulating Factor Rat Recombinant
Cat. No.
BT503
Source
Escherichia Coli.
Synonyms
Granulocyte colony stimulating factor, Protein Csf3, Csf3.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
(a)Analysis by RP-HPLC.
(b)Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished forLABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
GCSF Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids and having a molecular mass of 21.5kDa.
The G-CSF is purified by proprietary chromatographic techniques.
The G-CSF is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Granulocyte colony-stimulating factor (GCSF) is a cytokine that regulates the production, differentiation, and function of granulocytes. This cytokine is found in the extracellular matrix and exerts its effects outside of cells. The GCSF gene is known to have three different transcript variants, which encode three distinct isoforms of the protein. GCSF belongs to a family of cytokines known as granulocyte/macrophage colony-stimulating factors (CSFs). These CSFs play crucial roles in hematopoiesis, the process of blood cell formation, by regulating the production, differentiation, and function of two related white blood cell populations: granulocytes and monocytes-macrophages. As its name suggests, GCSF specifically stimulates the production of granulocytes.
Description
Recombinant Rat GCSF, produced in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 195 amino acids, resulting in a molecular mass of 21.5 kDa. The purification process of GCSF involves the use of proprietary chromatographic techniques.
Physical Appearance
Sterile Filtered White lyophilized powder.
Formulation
The product is provided as a lyophilized powder, which has been freeze-dried from a 0.2 μm filtered solution containing 5mM Sodium Citrate at a pH of 4.0.
Solubility
To reconstitute the lyophilized GCSF, it is recommended to dissolve it in sterile 18M-cm H₂O at a concentration of at least 100 μg/ml. This solution can then be further diluted into other aqueous solutions as needed.
Stability
Lyophilized GCSF demonstrates stability at room temperature for a period of 3 weeks. However, for long-term storage, it is recommended to store the lyophilized product in a desiccated state below -18°C. After reconstitution, GCSF should be stored at 4°C and is stable for 2-7 days. For extended storage, it is recommended to store the reconstituted product below -18°C. To ensure optimal stability during storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Avoid repeated freeze-thaw cycles to maintain protein integrity and activity.
Purity
The purity of GCSF is determined to be greater than 97.0% using the following methods:
(a) Analysis by RP-HPLC (Reverse Phase High-Performance Liquid Chromatography).
(b) Analysis by SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).
(a) Analysis by RP-HPLC (Reverse Phase High-Performance Liquid Chromatography).
(b) Analysis by SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).
Biological Activity
The biological activity of GCSF is determined by a cell proliferation assay using murine NFS-60 cells. The ED₅₀, which represents the effective concentration required to achieve 50% of the maximum response, is determined to be less than 0.05 ng/ml. This corresponds to a specific activity of greater than 2.0 × 10⁷ IU/mg.
Synonyms
Granulocyte colony stimulating factor, Protein Csf3, Csf3.
Source
Escherichia Coli.
Amino Acid Sequence
KKIPLLTVSS LPPSLPLPRS FLLKSLEQVR KIQARNTELL EQLCATYKLC HPEELVLFGH SLGIPKASLS SCSSQALQQT KCLSQLHSGL FLYQGLLQAL AGISSELAPT LDMLHLDVDN FATTIWQQME SLGVAPTVQP TQSTMPIFTS AFQRRAGGVL VTSYLQSFLE TAHHALHHLP RPAQKHFPES LFISI.
Product Science Overview
Introduction
Granulocyte-Colony Stimulating Factor (G-CSF) is a glycoprotein that plays a crucial role in hematopoiesis by stimulating the bone marrow to produce granulocytes and stem cells and release them into the bloodstream . The recombinant form of G-CSF, including that derived from rats, is used extensively in research and therapeutic applications.
Structure and Function
G-CSF is a 19.6-kDa glycoprotein that functions as a cytokine and hormone . It is produced by various tissues and is essential for the proliferation and differentiation of granulocyte hematopoietic progenitors . The recombinant rat G-CSF is produced from a DNA sequence encoding the mature protein, ensuring it retains the biological activity of the native protein .
Biological Properties
G-CSF exerts several biological effects, including:
- Neuroprotective Activities: It induces neuronal regeneration, inhibits neuronal apoptosis, mobilizes hematopoietic stem cells (HSCs), regulates pro and anti-inflammatory cytokines, and activates angiogenesis .
- Hematopoietic Effects: It stimulates the production of granulocytes and stem cells, which are crucial for the immune response and recovery from neutropenia .
Therapeutic Applications
Research and Development
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