GCP2 Bovine
Escherichia Coli.
C-X-C motif chemokine 6, Chemokine alpha 3, CKA-3, Granulocyte chemotactic protein 2, GCP-2, Small-inducible cytokine B6.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Granulocyte Chemotactic Protein 2 (CXCL6) Bovine Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 76 amino acids and having a molecular mass of approximately 8.0kDa.
GCP2 is purified by proprietary chromatographic techniques.
Product Specs
Granulocyte Chemotactic Protein 2 (CXCL6), also referred to as GCP-2, is a chemoattractant for neutrophil granulocytes. It functions by binding to and activating its receptors, CXCR1 and CXCR2. GCP-2 exhibits potent antibacterial effects against both Gram-positive and Gram-negative bacteria, in addition to its chemotactic and angiogenic properties.
Recombinant Bovine Granulocyte Chemotactic Protein 2 (CXCL6), produced in E. coli, is a non-glycosylated polypeptide chain composed of 76 amino acids with an approximate molecular weight of 8.0 kDa. The purification of GCP2 is achieved using proprietary chromatographic methods.
Sterile Filtered White lyophilized (freeze-dried) powder.
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB and 500 mM NaCl, pH 7.0.
For reconstitution of the lyophilized Granulocyte Chemotactic Protein 2 (CXCL6), it is recommended to use sterile 18 MΩ-cm H2O at a concentration not less than 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Lyophilized GCP2, while stable at room temperature for up to 3 weeks, should be stored desiccated at a temperature below -18 °C. After reconstitution, Granulocyte Chemotactic Protein 2 (CXCL6) should be stored at 4 °C for a period of 2-7 days. For long-term storage, it is recommended to store below -18 °C. It is important to avoid repeated freeze-thaw cycles.
The purity is determined to be greater than 97.0% using the following methods:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
The biological activity, as measured by a chemotaxis bioassay using human neutrophils, is in the range of 10-50 ng/ml.
C-X-C motif chemokine 6, Chemokine alpha 3, CKA-3, Granulocyte chemotactic protein 2, GCP-2, Small-inducible cytokine B6.
Escherichia Coli.
GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN.
Product Science Overview
The bovine recombinant form of CXCL6 is produced in Escherichia coli (E. coli) and is a non-glycosylated polypeptide chain containing 76 amino acids. It has a molecular mass of approximately 8.0 kDa . The recombinant protein is purified using proprietary chromatographic techniques to ensure high purity and biological activity .
CXCL6 exerts its chemotactic effects by interacting with the chemokine receptors CXCR1 and CXCR2 . It has strong antibacterial activity against both Gram-positive and Gram-negative bacteria, in addition to its chemotactic and angiogenic properties . The biological activity of CXCL6 is determined by a chemotaxis bioassay using human neutrophils, with an effective concentration range of 10-50 ng/ml .
The lyophilized form of CXCL6 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. It is important to avoid freeze-thaw cycles to maintain its stability and activity .
CXCL6 is used extensively in laboratory research to study its role in chemotaxis, immune response, and its potential therapeutic applications in inflammation and cancer . Its ability to recruit neutrophils and its antibacterial properties make it a valuable tool in understanding immune mechanisms and developing new treatments.
