GABARAP Human

GABA(A) Receptor-Associated Protein Human Recombinant

Cat. No.

BT10065

Source

Escherichia Coli.

Synonyms

GBRAP, MM46, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein, GABARAP, FLJ25768, MGC120154, MGC120155, FLC3B.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

GABARAP Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 137 amino acids (1-117 a.a.) and having a molecular mass of 16 kDa. GABARAP is fused to a 20 amino acid His-Tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

GABARAP, an abbreviation for GABA(A) receptor-associated protein, functions as a ligand-gated chloride channel crucial for inhibitory neurotransmission. Characterized by a highly positive charge at its N-terminus, GABARAP exhibits sequence homology with light chain-3 found in microtubule-associated proteins 1A and 1B. Its role involves clustering neurotransmitter receptors by facilitating interactions with the cytoskeleton.

Description

Recombinant human GABARAP, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 137 amino acids (specifically, amino acids 1 to 117). It possesses a molecular mass of 16 kDa. A 20 amino acid His-Tag is fused to the N-terminus of GABARAP. Purification is achieved using proprietary chromatographic methods.

Physical Appearance

A clear and colorless solution that has undergone sterile filtration.

Formulation

The GABARAP solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl at a pH of 8, 0.2 M NaCl, and 20% glycerol.

Stability

For short-term storage (up to 2-4 weeks), the GABARAP solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure long-term stability during storage, adding a carrier protein such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin) is advisable. Repeated freezing and thawing of the solution should be avoided.

Purity

Analysis by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) has determined the purity of GABARAP to be greater than 95%.

Synonyms

GBRAP, MM46, GABA(A) receptor-associated protein, Gamma-aminobutyric acid receptor-associated protein, GABARAP, FLJ25768, MGC120154, MGC120155, FLC3B.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL.

Product Science Overview

Introduction

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system (CNS). It plays a crucial role in regulating neuronal excitability throughout the nervous system. GABA exerts its effects through two main types of receptors: GABA(A) and GABA(B) receptors. Among these, GABA(A) receptors are the most prominent and are ligand-gated ion channels that mediate fast synaptic inhibition.

Structure and Function of GABA(A) Receptors

GABA(A) receptors are heteropentameric complexes composed of various subunits. There are 19 known subunits that can combine to form these receptors: six α (alpha1-6), three β (beta1-3), three γ (gamma1-3), three ρ (rho1-3), and one each of the δ (delta), ε (epsilon), π (pi), and θ (theta) subunits. The most common configuration in the human brain consists of two α subunits, two β subunits, and one γ subunit arranged in a specific manner around a central chloride ion channel.

GABA(A) Receptor-Associated Protein (GABARAP)

GABA(A) Receptor-Associated Protein (GABARAP) is a protein that plays a critical role in the trafficking and function of GABA(A) receptors. It is encoded by the GABARAP gene in the human genome. GABARAP is involved in the intracellular transport of GABA(A) receptors to the cell membrane, where they can participate in synaptic transmission. This protein is essential for the proper localization and function of GABA(A) receptors, thereby influencing inhibitory neurotransmission in the CNS.

Biological Significance

The proper functioning of GABA(A) receptors is vital for maintaining the balance between excitation and inhibition in the brain. Dysregulation of GABAergic signaling has been implicated in various neurological and psychiatric disorders, including epilepsy, anxiety, insomnia, and schizophrenia. GABARAP, by ensuring the correct trafficking and localization of GABA(A) receptors, plays a crucial role in maintaining this balance and, consequently, normal brain function.

Therapeutic Implications

Given the importance of GABA(A) receptors in various CNS disorders, GABARAP has emerged as a potential therapeutic target. Modulating the function or expression of GABARAP could influence the availability and activity of GABA(A) receptors, offering new avenues for the treatment of conditions associated with GABAergic dysfunction. Research is ongoing to develop compounds that can specifically target GABARAP and modulate its activity for therapeutic benefit.

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GABARAP Human

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