G CSF Antibody, FITC

Granulocyte Colony Stimulating Factor, Mouse Anti-Human, FITC
Cat. No.
BT5487
Source
Synonyms
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock

Description

Product Specs

Introduction
Granulocyte colony-stimulating factor (G-CSF) is a cytokine that plays a crucial role in the production, differentiation, and function of granulocytes, a type of white blood cell. This cytokine exerts its effects outside of cells (extracellularly). The gene encoding G-CSF has three identified transcript variants, which give rise to three distinct isoforms of the protein. G-CSF belongs to a family of cytokines known as granulocyte/macrophage colony-stimulating factors (CSFs), which are instrumental in hematopoiesis—the process of blood cell formation. These CSFs regulate the production, differentiation, and function of two closely related white blood cell populations: granulocytes and monocytes-macrophages. As its name suggests, G-CSF specifically stimulates the production of granulocytes.
Formulation
The antibody is supplied at a concentration of 1 milligram per milliliter (mg/ml) in phosphate-buffered saline (PBS) after reconstitution.
Shipping Conditions
The antibody is shipped in a lyophilized (freeze-dried) form at ambient temperature.
Storage Procedures
For long-term storage, keep the lyophilized antibody at 4 degrees Celsius in a dry environment. After reconstitution, if the antibody is not intended for use within one month, it is recommended to aliquot (divide into smaller portions) and store at -20 degrees Celsius.
Solubility
To reconstitute the antibody, add sterile water (H2O). Mix gently by swirling, ensure that the sides of the vial are washed, and allow 30-60 seconds for complete reconstitution before use.
Applications
This antibody is suitable for use in various applications, including direct enzyme-linked immunosorbent assay (ELISA), Western blotting, and intracellular staining.
Titer
For intracellular staining, the recommended dilution is 10 microliters (µl) of antibody per 1,000,000 cells.
Synonyms
CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Purification Method
Protein A.
Type
Mouse Anti Human Monoclonal.
Clone
NYRhGCSF.
Immunogen
r.Human G-CSF.
Ig Subclass
Mouse IgG.

Product Science Overview

Mouse Anti-Human G-CSF Antibody

Mouse anti-human G-CSF antibodies are monoclonal antibodies produced by immunizing mice with human G-CSF. These antibodies are highly specific and can be used in various research and diagnostic applications, including enzyme-linked immunosorbent assays (ELISA), western blotting, and flow cytometry. They are valuable tools for studying the expression, function, and regulation of G-CSF in different biological contexts .

FITC Labeling

Fluorescein isothiocyanate (FITC) is a fluorescent dye commonly used to label antibodies and other biomolecules. FITC-labeled antibodies can be detected using fluorescence microscopy or flow cytometry, allowing researchers to visualize and quantify the presence of specific antigens in cells or tissues. FITC emits green fluorescence when excited by blue light, making it a popular choice for multi-color staining experiments .

Applications
  1. Research: FITC-labeled mouse anti-human G-CSF antibodies are widely used in research to study the role of G-CSF in various physiological and pathological processes. They can help elucidate the mechanisms underlying G-CSF-mediated signaling pathways and its effects on immune cell function and development .

  2. Clinical Diagnostics: These antibodies can be used in diagnostic assays to measure G-CSF levels in patient samples. This is particularly useful for monitoring patients undergoing chemotherapy or bone marrow transplantation, as G-CSF levels can provide valuable information about their immune status and response to treatment .

  3. Therapeutic Development: Understanding the interaction between G-CSF and its receptor can aid in the development of new therapeutic strategies for conditions involving neutropenia or other immune-related disorders. FITC-labeled antibodies can be used to screen for potential drug candidates that modulate G-CSF activity .

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