FRZB Human

Frizzled-Related Protein Human Recombinant
Cat. No.
BT10180
Source
Escherichia Coli.
Synonyms
Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, FRZB, FIZ, FRE, FRP, FRZB1, SFRP3, OS1, FZRB, hFIZ, FRP-3, SFRP3, SRFP3, FRZB-PEN.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FRZB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 316 amino acids (33-325 a.a) and having a molecular mass of 35kDa.
FRZB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Frizzled-Related Protein (FRZB) is a secreted protein that plays a role in regulating bone development. Genetic defects in the FRZB gene are linked to an increased susceptibility to osteoarthritis (OA), particularly in females. FRZB belongs to the soluble frizzled-related protein (sFRP) family, which modulate Wnt signaling pathways by directly interacting with Wnt proteins. sFRPs are essential for regulating cell growth and differentiation in specific cell types. Notably, SFRP3/FRZB is involved in the development of limb skeletons. FRZB also regulates the maturation of chondrocytes and the growth of long bones.
Description
Recombinant human FRZB protein, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 316 amino acids (residues 33-325). It has a molecular weight of 35 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The FRZB protein solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the FRZB protein solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal protein stability during long-term storage, adding a carrier protein such as 0.1% HSA or BSA is advisable. Repeated freezing and thawing of the protein solution should be avoided.
Purity
The purity of the FRZB protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, FRZB, FIZ, FRE, FRP, FRZB1, SFRP3, OS1, FZRB, hFIZ, FRP-3, SFRP3, SRFP3, FRZB-PEN.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSAACEPVR IPLCKSLPWN MTKMPNHLHH STQANAILAI EQFEGLLGTH CSPDLLFFLC AMYAPICTID FQHEPIKPCK SVCERARQGC EPILIKYRHS WPENLACEEL PVYDRGVCIS PEAIVTADGA DFPMDSSNGN CRGASSERCK CKPIRATQKT YFRNNYNYVI RAKVKEIKTK CHDVTAVVEV KEILKSSLVN IPRDTVNLYT SSGCLCPPLN VNEEYIIMGY EDEERSRLLL VEGSIAEKWK DRLGKKVKRW DMKLRHLGLS KSDSSNSDST QSQKSGRNSN PRQARN.

Product Science Overview

Structure and Function

Frizzled-related proteins contain a cysteine-rich domain (CRD) that is homologous to the Wnt-binding domain of Frizzled receptors . This domain enables FRPs to bind to Wnt ligands or Frizzled receptors, thereby modulating Wnt signaling . The human recombinant version of these proteins is produced using recombinant DNA technology, which involves inserting the gene encoding the protein into a suitable expression system, such as bacteria or mammalian cells, to produce the protein in large quantities .

Role in Wnt Signaling

The Wnt signaling pathway can be divided into two main branches: the canonical (β-catenin-dependent) pathway and the non-canonical (β-catenin-independent) pathway . FRPs can act as antagonists of the Wnt signaling pathway by binding to Wnt ligands and preventing them from interacting with Frizzled receptors . This inhibition can affect various cellular processes, including cell proliferation, differentiation, and migration .

Applications in Research and Medicine

Recombinant human FRPs are valuable tools in research and medicine due to their ability to modulate Wnt signaling . They are used in various applications, including:

  • Cancer Research: FRPs have been shown to play a role in tumor angiogenesis, making them potential targets for anti-cancer therapies .
  • Developmental Biology: Studying the role of FRPs in embryonic development can provide insights into the mechanisms underlying various developmental disorders .
  • Regenerative Medicine: Modulating Wnt signaling using FRPs can promote tissue regeneration and repair .

In conclusion, Frizzled-related proteins are essential regulators of the Wnt signaling pathway, with significant implications for developmental biology, cancer research, and regenerative medicine. The recombinant production of these proteins has enabled their widespread use in various research and therapeutic applications.

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