FRZB Human, Sf9
Sf9, Baculovirus cells.
Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, FRZB, FIZ, FRE, FRP, FRZB1, SFRP3, OS1, FZRB, hFIZ, FRP-3, SFRP3, SRFP3, FRZB-PEN.
Greater than 90% as determined by SDS-PAGE.
Description
FRZB produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 302 amino acids (33-325 a.a.) and having a molecular mass of 34.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
FRZB is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Frizzled-Related Protein (FRZB), a secreted protein, plays a crucial role in regulating bone development. Genetic defects in the FRZB gene are linked to a higher susceptibility to osteoarthritis (OA), particularly in females. Soluble frizzled-related proteins (sFRPS), including FRZB, modulate Wnt signaling pathways by directly interacting with Wnt proteins. These sFRPS are essential for controlling cell growth and differentiation in specific cell types. FRZB, also known as SFRP3, is involved in limb skeletogenesis, regulating chondrocyte maturation, and long bone development.
Produced in Sf9 insect cells, FRZB is a single, glycosylated polypeptide chain consisting of 302 amino acids (33-325 a.a.). It has a molecular mass of 34.2kDa. Note that on SDS-PAGE, the apparent molecular size might appear between 28-40kDa. This FRZB protein is expressed with a 9 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
The FRZB solution is provided at a concentration of 0.25mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
The purity of FRZB is greater than 90% as determined by SDS-PAGE analysis.
Secreted frizzled-related protein 3, sFRP-3, Frezzled, Fritz, Frizzled-related protein 1, FrzB-1, FRZB, FIZ, FRE, FRP, FRZB1, SFRP3, OS1, FZRB, hFIZ, FRP-3, SFRP3, SRFP3, FRZB-PEN.
Sf9, Baculovirus cells.
ADPAACEPVR IPLCKSLPWN MTKMPNHLHH STQANAILAI EQFEGLLGTH CSPDLLFFLC AMYAPICTID FQHEPIKPCK SVCERARQGC EPILIKYRHS WPENLACEEL PVYDRGVCIS PEAIVTADGA DFPMDSSNGN CRGASSERCK CKPIRATQKT YFRNNYNYVI RAKVKEIKTK CHDVTAVVEV KEILKSSLVN IPRDTVNLYT SSGCLCPPLN VNEEYIIMGY EDEERSRLLL VEGSIAEKWK DRLGKKVKRW DMKLRHLGLS KSDSSNSDST QSQKSGRNSN PRQARNHHHH HH.
Product Science Overview
FRZB is a secreted glycoprotein that consists of 302 amino acids and has a molecular mass of approximately 34.2 kDa . The recombinant form of FRZB is produced in Sf9 insect cells using the baculovirus expression system. This recombinant protein is expressed with a 9 amino acid His tag at the C-terminus, which facilitates its purification through chromatographic techniques .
FRZB functions as a modulator of the Wnt signaling pathway by directly interacting with Wnt proteins. This interaction inhibits the Wnt signaling, which is crucial for regulating cell growth and differentiation in specific cell types . FRZB is particularly involved in the regulation of bone development and chondrocyte maturation. It plays a significant role in limb skeletogenesis and long bone development .
The recombinant FRZB protein produced in Sf9 insect cells is a single, glycosylated polypeptide chain. The production process involves the use of baculovirus vectors to infect the Sf9 cells, leading to the expression of the FRZB protein. The protein is then purified using proprietary chromatographic techniques to achieve a purity level greater than 90% as determined by SDS-PAGE .
The FRZB protein solution is formulated in phosphate-buffered saline (PBS) with 10% glycerol and is sterile-filtered to ensure its stability and sterility. For short-term storage, the protein can be kept at 4°C for 2-4 weeks. For long-term storage, it is recommended to store the protein at -20°C with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation .
