Sterile Filtered colorless solution.
Greater than 90% as determined by SDS-PAGE.
Flagellin Listeria Monocytogenes Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of approximately 31.2kDa. The Flagellin FLA is fused to a 6xHis tag at N-terminus and purified by proprietary chromatographic techniques.
Listeria monocytogenes is a Gram-positive, facultative intracellular bacterium that is widely recognized as a foodborne pathogen responsible for listeriosis. This bacterium is known for its ability to thrive in a variety of environments, including refrigerated foods, making it a significant concern for food safety. One of the key features of Listeria monocytogenes is its motility, which is facilitated by the presence of flagella.
Flagellin is the primary structural protein of bacterial flagella, which are whip-like appendages that enable bacterial motility. In Listeria monocytogenes, the gene encoding flagellin is known as flaA. The flagellin protein is crucial for the bacterium’s ability to move and colonize various surfaces, including plant tissues and host cells .
Recombinant flagellin refers to the flagellin protein that has been produced using recombinant DNA technology. This involves cloning the flaA gene from Listeria monocytogenes into a suitable expression system, such as Escherichia coli, to produce the flagellin protein in large quantities. The recombinant flagellin protein can be tagged with a polyhistidine (His) tag to facilitate purification and detection .
The recombinant flagellin protein retains the biological properties of the native flagellin. It plays a critical role in the motility of Listeria monocytogenes, allowing the bacterium to move towards favorable environments and away from hostile conditions. Additionally, flagellin is involved in the attachment and colonization of surfaces, which is essential for the bacterium’s ability to infect host tissues .
The expression of flagellin in Listeria monocytogenes is tightly regulated by several factors. One of the key regulators is the transcriptional repressor MogR, which represses the expression of flagellin during extracellular growth at 37°C and during intracellular infection. This regulation is crucial for the bacterium’s virulence and ability to evade the host immune response .
Recombinant flagellin has several applications in research and biotechnology. It can be used to study the mechanisms of bacterial motility and host-pathogen interactions. Additionally, recombinant flagellin can be employed in the development of vaccines and diagnostic tools, as it is a potent activator of the innate immune system.