Flagellin

Flagellin Recombinant
Cat. No.
BT9484
Source
Escherichia Coli.
Synonyms
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Flagellin Salmonella typhimurium Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids with Leu, Glu and a 6 × His at C-terminus and having a molecular mass of 52.7kDa.
The Flagellin is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Flagellin is a protein that forms the filament of bacterial flagella, which are whip-like structures bacteria use for movement. It is a major component of flagella and is found in large amounts on nearly all bacteria that have them.
Description
Recombinant Flagellin from Salmonella typhimurium, produced in E. coli, is a single polypeptide chain that lacks glycosylation. It contains 503 amino acids, including Leucine, Glutamic acid, and a 6x Histidine tag at the C-terminus. The molecular weight is 52.7 kDa. This Flagellin has been purified using proprietary chromatographic methods.
Physical Appearance
White powder that has been sterilized by filtration and freeze-dried.
Formulation
Freeze-dried from a 0.2 micrometer filtered solution concentrated in phosphate buffered saline (PBS) at a pH of 7.4.
Solubility
To reconstitute the freeze-dried Flagellin, it is recommended to dissolve it in sterile 18 megaohm-centimeter H₂O at a concentration of at least 100 micrograms per milliliter. This solution can be further diluted into other aqueous solutions.
Stability
Lyophilized Flagellin remains stable for 3 weeks when stored at room temperature. However, it is best to store it in a dry environment below -18°C. After reconstitution, Flagellin can be stored at 4°C for 2 to 7 days. For longer storage, keep it below -18°C. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE and HPLC analysis.
Source
Escherichia Coli.
Amino Acid Sequence
MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLRLEHHH HHH

Product Science Overview

Structure and Function

Flagellin is composed of four domains: D0, D1, D2, and D3. The D0 and D1 domains are the most conserved and are critical for the protein’s structural integrity and function . The D2 and D3 domains are more variable and are involved in the immune recognition of flagellin .

Immune Recognition

Flagellin is recognized by the host immune system as a microbe-associated molecular pattern (MAMP). It is detected by Toll-like receptor 5 (TLR5) on the cell surface and by NLR neuronal apoptosis inhibitory protein 5 (NAIP5) and NAIP6 in the cytosol . The interaction with TLR5 triggers the activation of pro-inflammatory genes, including IL-6 and TNF-α, which are essential for mounting an immune response .

Recombinant Flagellin

Recombinant flagellin refers to flagellin that has been produced using recombinant DNA technology. This involves inserting the gene encoding flagellin into a suitable expression system, such as bacteria or yeast, to produce the protein in large quantities . Recombinant flagellin retains the immunostimulatory properties of the native protein and is used in various research and therapeutic applications .

Applications
  1. Vaccine Adjuvant: Recombinant flagellin is used as an adjuvant in vaccines to enhance the immune response. It has been shown to improve the efficacy of vaccines against various pathogens by promoting the activation of both innate and adaptive immunity .
  2. Cancer Therapy: Flagellin has been investigated for its potential use in cancer therapy. It can stimulate the immune system to recognize and attack tumor cells, making it a promising candidate for immunotherapy .
  3. Research Tool: In research, recombinant flagellin is used to study the mechanisms of immune recognition and response. It serves as a model antigen to investigate the interactions between bacterial components and the host immune system .

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Source
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