FLAG-HRP Antibody

The FLAG peptide is a short, hydrophilic peptide consisting of eight amino acids: Aspartic acid (D), Tyrosine (Y), Lysine (K), Aspartic acid (D), Aspartic acid (D), Aspartic acid (D), Aspartic acid (D), and Lysine (K), represented by the sequence DYKDDDDK . This peptide is widely used as a fusion tag for the purification and detection of recombinant proteins.

The FLAG peptide is specifically designed for immunoaffinity chromatography, allowing for the elution of fusion proteins under non-denaturing conditions . It is commonly used in various biochemical and molecular biology techniques, including:

• Western Blotting: For detecting FLAG-tagged proteins.
• Immunocytochemistry: For visualizing the localization of FLAG-tagged proteins within cells.
• Immunoprecipitation: For isolating FLAG-tagged proteins from complex mixtures.
• Flow Cytometry: For analyzing the expression of FLAG-tagged proteins on the cell surface.
• Protein Purification: For purifying FLAG-tagged proteins using affinity chromatography .

The monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase (HRP) . This conjugation allows for the detection of FLAG fusion proteins through enzymatic reactions that produce a colorimetric or chemiluminescent signal. The antibody recognizes the FLAG epitope at various positions, including N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides .

The ANTI-FLAG M2-Peroxidase antibody is typically supplied in a buffered aqueous glycerol solution and is used in various applications such as:

• Immunocytochemistry: Suggested dilution of 1:100 to 1:1000.
• Western Blotting: Suggested dilution of 1:100 to 1:1000.
• ELISA: Suggested dilution of 1:20,000 .

For optimal results, the antibody solution should be diluted in Tris Buffered Saline (TBS) with a pH of 7.4 and 0.15 M NaCl .