FLAG PAT3G6AT antibody
Description
Product Specs
The FLAG tag is an eight amino acid peptide sequence (AspTyrLysAspAspAspAspLys) that includes an enterokinase-cleavage site. It is specifically designed for immunoaffinity chromatography, enabling elution under non-denaturing conditions. Several antibodies have been developed against the FLAG peptide, including M1, which requires the presence of bivalent metal cations, preferably Ca(+), for binding. Chelating agents are used for elution with the M1 antibody. Another strategy employs competitive elution using an excess of free FLAG Peptide. The FLAG peptide is a versatile tool for purifying and detecting recombinant fusion proteins, proving useful in various applications like Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, and protein purification. Moreover, it aids in studying protein-protein interactions, cell ultrastructure, and protein localization. As a hydrophilic tag, the FLAG peptide significantly enhances the detection and purification of recombinant fusion proteins.
The solution is at a concentration of 1mg/ml and contains PBS at a pH of 7.4, 10% Glycerol, and 0.02% Sodium Azide.
FLAG antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT3G6AT.
Anti-human FLAG mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a Synthetic peptide (DYKDDDDKC)-KLH.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
The FLAG peptide is a widely used fusion tag in molecular biology and biochemistry. It consists of eight amino acids (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) and includes an enterokinase-cleavage site. This peptide is specifically designed for immunoaffinity chromatography, allowing elution under non-denaturing conditions .
The FLAG peptide and its corresponding antibodies, such as Clone PAT3G6AT, are highly versatile and are used in various applications, including:
- Western Blotting: Detecting proteins separated by gel electrophoresis.
- Immunocytochemistry (ICC): Visualizing the localization of proteins within cells.
- Immunoprecipitation (IP): Isolating proteins from a mixture using an antibody.
- Flow Cytometry: Analyzing the physical and chemical characteristics of cells or particles.
- Protein Purification: Isolating recombinant fusion proteins.
- Protein-Protein Interaction Studies: Investigating interactions between proteins.
- Cell Ultrastructure Studies: Examining the detailed structure of cells.
- Protein Localization Studies: Determining the specific location of proteins within cells .
The FLAG antibody is formulated at a concentration of 1 mg/ml in PBS (pH 7.4) containing 10% glycerol and 0.02% sodium azide. For optimal stability, it should be stored at -20°C for long-term storage and at 4°C for short-term storage (up to 1 month). It is important to prevent freeze-thaw cycles to maintain the antibody’s integrity .
