FKBP5 Human

FK506 Binding Protein 5 Human Recombinant
Cat. No.
BT20660
Source
Escherichia Coli.
Synonyms
FK506 Binding Protein 5, 54 KDa Progesterone Receptor-Associated Immunophilin, 51 KDa FK506-Binding Protein, Androgen-Regulated Protein 6, HSP90-Binding Immunophilin, FK506-Binding Protein 5, PPIase FKBP5, 51 KDa FKBP, FF1 Antigen, EC 5.2.1.8, Rotamase, FKBP-51, FKBP51, FKBP54, AIG6, P54, Peptidylprolyl Cis-Trans Isomerase, T-Cell FK506-Binding Protein, PPIASE, Ptg-10, FKBP-5, FKBP5.
Appearance
Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FKBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Met1-Val457) containing 467 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 52.5kDa.

Product Specs

Introduction
FK506 Binding Protein 5, also known as FKBP5, is part of the immunophilin protein family. This family plays a crucial role in regulating immune responses and essential cellular functions like protein folding and transport. FKBP5 acts as a cis-trans prolyl isomerase, binding to immunosuppressive drugs like FK506 and rapamycin. Its functions include PPIase and co-chaperone activities, and it is believed to be involved in inhibiting calcineurin. FKBP5 interacts with heat-shock protein 90 (HSP90), forming complexes with unligated steroid receptors. Additionally, it interacts with mature hetero-oligomeric progesterone receptor complexes alongside HSP90 and P23 protein.
Description
Recombinant human FKBP5 protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 52.5kDa. The protein sequence consists of 467 amino acids, including a 10 amino acid His tag located at the N-terminus (Met1-Val457).
Physical Appearance
White powder, lyophilized (freeze-dried) and filtered.
Formulation
The FKBP5 protein was lyophilized in a 20mM Tris buffer with 50mM NaCl at pH 7.5, and then filtered through a 0.4µm filter.
Solubility
To prepare a working stock solution, add deionized water to the lyophilized pellet aiming for a concentration of approximately 0.5mg/ml. Ensure the pellet completely dissolves. Before using the non-sterile FKBP5 in cell culture, it is crucial to filter sterilize the culture media or working solutions containing it.
Stability
For long-term storage, keep the lyophilized protein at -20°C. After reconstitution, aliquot the product to prevent repeated freeze-thaw cycles. Reconstituted protein remains stable at 4°C for a limited time, showing no changes for up to two weeks.
Purity
SDS-PAGE analysis confirms a purity greater than 90.0%.
Synonyms
FK506 Binding Protein 5, 54 KDa Progesterone Receptor-Associated Immunophilin, 51 KDa FK506-Binding Protein, Androgen-Regulated Protein 6, HSP90-Binding Immunophilin, FK506-Binding Protein 5, PPIase FKBP5, 51 KDa FKBP, FF1 Antigen, EC 5.2.1.8, Rotamase, FKBP-51, FKBP51, FKBP54, AIG6, P54, Peptidylprolyl Cis-Trans Isomerase, T-Cell FK506-Binding Protein, PPIASE, Ptg-10, FKBP-5, FKBP5.
Source
Escherichia Coli.
Amino Acid Sequence

MKHHHHHHAS MTTDEGAKNN EESPTATVAE QGEDITSKKD RGVLKIVKRV GNGEETPMIG DKVYVHYKGK LSNGKKFDSS HDRNEPFVFS LGKGQVIKAW DIGVATMKKG EICHLLCKPE YAYGSAGSLP KIPSNATLFF EIELLDFKGE DLFEDGGIIR RTKRKGEGYS NPNEGATVEI HLEGRCGGRM FDCRDVAFTV GEGEDHDIPI GIDKALEKMQ REEQCILYLG PRYGFGEAGK PKFGIEPNAE LIYEVTLKSF EKAKESWEMD TKEKLEQAAI VKEKGTVYFK GGKYMQAVIQ YGKIVSWLEM EYGLSEKESK ASESFLLAAF LNLAMCYLKL REYTKAVECC DKALGLDSAN EKGLYRRGEA QLLMNEFESA KGDFEKVLEV NPQNKAARLQ ISMCQKKAKE HNERDRRIYA NMFKKFAEQD AKEEANKAMG KKTSEGVTNE KGTDSQAMEE EKPEGHV.

Product Science Overview

Structure and Function

FKBP5 is a cis-trans prolyl isomerase that binds to the immunosuppressants tacrolimus (FK506) and sirolimus (rapamycin) . It mediates calcineurin inhibition, which is essential for its immunosuppressive properties . FKBP5 also interacts functionally with mature corticoid receptor hetero-complexes, including progesterone, glucocorticoid, and mineralocorticoid receptor complexes, along with the 90 kDa heat shock protein (Hsp90) and PTGES3 (P23 protein) .

As an Hsp90-associated co-chaperone, FKBP5 regulates the responsiveness of steroid hormone receptors, playing a significant role in stress endocrinology and glucocorticoid signaling .

Clinical Significance

The FKBP5 gene has been found to have multiple polyadenylation sites and is statistically associated with a higher rate of depressive disorders . Decreased methylation in the promoter of the FKBP5 gene has been observed in blood samples from patients with neurodegenerative diseases .

Therapeutic Potential

FKBP5 is an attractive drug target due to its involvement in several diseases, including stress-related disorders, chronic pain, and obesity . SAFit2, a well-characterized FKBP51 ligand, has shown promising effects in numerous animal models . Macrocyclic FKBP51-selective ligands are non-immunosuppressive, engage FKBP51 in cells, and block the cellular effect of FKBP51 .

Evolutionary Perspective

Comparative analysis of FKBP family proteins reveals that the eight known Drosophila FKBPs share homology with the human FKBP12, indicating a close evolutionary relationship . The known FKBPs contain FK domains, which are prolyl cis/trans isomerase (PPIase) domains that mediate immune suppression through inhibition of calcineurin .

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