FKBP4 Antibody
FK506 Binding Protein 4, Mouse Anti Human
Cat. No.
BT17322
Source
Synonyms
HBI, p52, Hsp56, FKBP52, FKBP59, PPIase, FKBP4, FK506-binding protein 4, Peptidyl-prolyl cis-trans isomerase, HSP-binding immunophilin, FKBP52 protein, 52 kDa FK506-binding protein, p59 protein.
Appearance
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Product Specs
Introduction
Belonging to the immunophilin protein family, FKBP4 plays a crucial role in immunoregulation and essential cellular processes like protein folding and trafficking. This cis-trans prolyl isomerase interacts with immunosuppressants such as FK506 and rapamycin. Despite its structural and functional similarities to FKBP1A, FKBP4 does not exhibit immunosuppressant activity when forming a complex with FK506. It interacts with interferon regulatory factor-4, contributing to immunoregulatory gene expression in B and T lymphocytes. Additionally, FKBP4 interacts with phytanoyl-CoA alpha-hydroxylase and associates with HSP90 and HSP70, participating in the intracellular trafficking of hetero-oligomeric forms of steroid hormone receptors. Its strong association with adeno-associated virus type 2 vectors (AAV) leads to a significant enhancement of AAV-mediated transgene expression in human cell lines, highlighting its role in optimizing AAV vectors for human gene therapy.
Formulation
The antibody is supplied at a concentration of 1mg/ml and is formulated in a solution of PBS at a pH of 7.4, with 0.1% Sodium Azide.
Storage Procedures
For short-term storage of up to one month, the antibody should be kept at a temperature of 4°C. For prolonged storage, it is recommended to store the antibody at -20°C. It is important to avoid repeated freeze-thaw cycles to maintain antibody stability.
Stability / Shelf Life
The antibody remains stable for a period of 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This FKBP4 antibody has been rigorously tested across various applications, including ELISA, Western blot, and immunohistochemistry analysis, to ensure its specificity and reactivity. However, it is essential to note that optimal working dilutions may vary depending on the specific application and experimental conditions. For Western blot analysis, a dilution range of 1:1,000 to 1:2,000 is recommended, with a starting dilution of 1:1,000. For immunohistochemistry analysis, a dilution range of 1:50 to 1:100 is suggested, starting with a dilution of 1:50. Titration is always advised for each investigation to determine the most effective working concentration.
Synonyms
HBI, p52, Hsp56, FKBP52, FKBP59, PPIase, FKBP4, FK506-binding protein 4, Peptidyl-prolyl cis-trans isomerase, HSP-binding immunophilin, FKBP52 protein, 52 kDa FK506-binding protein, p59 protein.
Purification Method
FKBP4 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT4D3AT.
Immunogen
Anti-human FKBP4 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human FKBP4 amino acids 1-459 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.
Product Science Overview
Introduction
FK506 Binding Protein 4 (FKBP4), also known as FKBP52, is a member of the immunophilin protein family. These proteins are characterized by their ability to bind to immunosuppressive drugs such as FK506 (tacrolimus) and rapamycin. FKBP4 is a highly conserved protein that plays a crucial role in various cellular processes, including protein folding, trafficking, and signal transduction.
Structure and Function
FKBP4 contains several functional domains:
- FKBP Domain: This domain exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is essential for protein folding.
- Tetratricopeptide Repeat (TPR) Domains: These domains facilitate protein-protein interactions, particularly with heat shock proteins (Hsp90 and Hsp70), forming a complex that is vital for the proper functioning of steroid hormone receptors .
Role in Cellular Processes
FKBP4 is involved in multiple cellular processes:
- Steroid Receptor Signaling: FKBP4 acts as a co-chaperone with Hsp90, aiding in the proper folding and function of steroid hormone receptors such as the glucocorticoid receptor and androgen receptor .
- Protein Trafficking: FKBP4 is implicated in the trafficking of proteins within the cell, ensuring they reach their correct cellular compartments .
- Neuroprotection: FKBP4 has been shown to play a role in protecting neurons from stress-induced damage, highlighting its potential therapeutic importance in neurodegenerative diseases .
Mouse Anti Human FKBP4 Antibodies
Mouse anti-human FKBP4 antibodies are monoclonal antibodies developed to specifically target and bind to human FKBP4. These antibodies are widely used in research and diagnostic applications, including:
Research and Clinical Implications
Research on FKBP4 has provided insights into its role in various diseases:
- Cancer: FKBP4 is implicated in the progression of certain cancers, making it a potential target for cancer therapy .
- Neurodegenerative Diseases: Given its neuroprotective role, FKBP4 is being studied for its potential in treating conditions like Alzheimer’s and Parkinson’s disease .
- Inflammatory Diseases: FKBP4’s involvement in steroid receptor signaling suggests it may play a role in inflammatory responses, offering another avenue for therapeutic intervention .
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