FIS1 Human

FIS1 is a single-pass membrane protein that contains one Tetratricopeptide Repeat (TPR). It is a member of the FIS1 family and is characterized by its ability to integrate into the mitochondrial outer membrane. The protein has a calculated molecular weight of approximately 15.3 kDa .

FIS1 is a key component of the mitochondrial complex that promotes mitochondrial fission. This process is vital for several cellular functions, including:

• Mitochondrial Distribution: Ensuring proper distribution of mitochondria during cell division.
• Quality Control: Removing damaged mitochondria through a process called mitophagy.
• Apoptosis: FIS1 can induce the release of cytochrome C from the mitochondria to the cytosol, leading to programmed cell death .

Additionally, FIS1 is involved in the growth and division of peroxisomes, another type of organelle involved in cellular metabolism .

Recombinant Human FIS1 is produced using E. coli expression systems. The recombinant form is often tagged with a His-tag to facilitate purification and detection. The protein is typically lyophilized from a solution containing Tris-HCl and other stabilizers to ensure its stability during storage and transport .

Recombinant FIS1 is widely used in research to study mitochondrial dynamics, apoptosis, and related cellular processes. It is also utilized in drug discovery and development, particularly in the context of diseases associated with mitochondrial dysfunction.

Lyophilized recombinant FIS1 is stable for up to 12 months when stored at -20 to -80°C. Once reconstituted, the protein solution can be stored at 4-8°C for 2-7 days or at -20°C for up to 3 months .