FIS1 Antibody
TTC11, Tetratricopeptide repeat domain 11, Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae).
Description
Product Specs
TTC11, Tetratricopeptide repeat domain 11, Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae).
FIS1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
PAT3B7AT.
Anti-human FIS1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FIS1 protein 1-122 amino acids purified from E. coli.
Mouse IgG1 heavy chain and k light chain.
Product Science Overview
MFission-1 is a monoclonal antibody derived from mice, specifically designed to target human antigens. Monoclonal antibodies are laboratory-produced molecules engineered to serve as substitute antibodies that can restore, enhance, or mimic the immune system’s attack on cells. They are highly specific, binding to a single epitope on an antigen.
The development of MFission-1 involves immunizing mice with a specific human antigen. The immune response in the mice generates B cells that produce antibodies against the human antigen. These B cells are then fused with myeloma cells to create hybridoma cells, which can be cultured to produce large quantities of the monoclonal antibody. The hybridoma technology, pioneered by Georges Köhler and César Milstein in 1975, is a cornerstone of monoclonal antibody production.
MFission-1, like other monoclonal antibodies, works by specifically binding to its target antigen on human cells. This binding can have several effects:
- Neutralization: The antibody can neutralize the biological activity of the antigen.
- Opsonization: The antibody can mark the antigen for destruction by other immune cells.
- Complement Activation: The antibody can activate the complement system, leading to the lysis of the target cell.
- Antibody-Dependent Cellular Cytotoxicity (ADCC): The antibody can recruit immune cells to kill the target cell.
MFission-1 has a wide range of applications in both research and clinical settings:
- Research: It is used in various assays to study the expression and function of the target antigen. This includes techniques such as flow cytometry, immunohistochemistry, and Western blotting.
- Diagnostics: MFission-1 can be used in diagnostic tests to detect the presence of its target antigen in patient samples.
- Therapeutics: Monoclonal antibodies like MFission-1 are used in the treatment of diseases, including cancers, autoimmune disorders, and infectious diseases. They can be used alone or conjugated with drugs, toxins, or radioactive substances to deliver targeted therapy.
Advantages:
- Specificity: High specificity for the target antigen reduces off-target effects.
- Versatility: Can be used in various applications, from basic research to clinical therapy.
- Reproducibility: Monoclonal antibodies provide consistent results due to their uniformity.
Limitations:
- Production Cost: The production of monoclonal antibodies is expensive and time-consuming.
- Immunogenicity: As a mouse-derived antibody, MFission-1 may elicit an immune response in humans, potentially leading to reduced efficacy and adverse reactions.
- Limited Targets: Monoclonal antibodies are specific to a single epitope, which may limit their effectiveness against antigens with multiple epitopes or those that mutate frequently.
