Fibronectin Human
Filtered White lyophilized (freeze-dried) powder.
≥ 95.0% as determined by SDS-PAGE.
Description
Human Fibronectin produced purified from Human Plasma having a Molecular Weight of 440kDa.
Product Specs
Purified Human Fibronectin, derived from Human Plasma, with a Molecular Weight of 440kDa.
The product appears as a white, lyophilized (freeze-dried) powder after filtration.
For the purpose of lyophilization, the Fibronectin was prepared in a non-sterile solution containing 2mg/ml of Fibronectin, 10mM sodium phosphate buffer (pH 7.5), and 0.15M NaCl.
To reconstitute the 1mg Fibronectin, we recommend using a chaotropic agent such as urea. Dissolve the protein in sterile water at room temperature to achieve a concentration of 0.2mg/ml. A urea concentration of 4M-5M is advisable. Allow the solution to stand for 1-2 hours. When utilizing the protein for cell attachment, ensure to remove the urea by washing after the fibronectin has adhered to the growth surface (plate or dish).
Lyophilized Fibronectin demonstrates stability at room temperature for up to 3 weeks. However, for extended storage, it is recommended to store the desiccated product below -18°C. Upon reconstitution, Fibronectin can be stored at 4°C for 2-7 days. For long-term storage, it should be kept below -18°C.
The purity of the Fibronectin is ≥ 95.0% as determined by SDS-PAGE analysis.
Product Science Overview
Fibronectin is composed of two nearly identical polypeptide chains linked by a pair of disulfide bonds at their C-termini . Each chain has a molecular weight of approximately 230-275 kDa and contains three types of repeating modules: type I, type II, and type III . The fibronectin gene is located on human chromosome 2q34-36 and spans 75kb, containing 50 exons . Alternative splicing of its pre-mRNA leads to the creation of several isoforms, allowing fibronectin to perform a variety of functions in different tissues .
Fibronectin is involved in numerous biological processes:
- Cell Adhesion and Migration: Fibronectin binds to integrins, which are membrane-spanning receptor proteins, facilitating cell adhesion and migration . This interaction is essential for wound healing and embryonic development .
- Tissue Repair: During tissue repair, plasma fibronectin is incorporated into fibrin clots to mediate hemostasis and platelet function . Cellular fibronectin is then synthesized and assembled by cells to reconstitute damaged tissue .
- Extracellular Matrix Organization: Fibronectin acts as a structural scaffold, maintaining and directing tissue organization and extracellular matrix composition .
- Regulation of Cellular Processes: Fibronectin regulates various cellular processes, including growth, differentiation, and migration .
Altered fibronectin expression, degradation, and organization have been associated with several pathologies, including cancer, arthritis, and fibrosis . Understanding the mechanisms involved in fibronectin assembly and its role in tissue repair can reveal potential therapeutic targets for treating aberrant tissue-repair conditions .
