FGFR2 Human, (22-289)

The fibroblast growth factor (FGF) family consists of at least 18 structurally related proteins involved in various physiological and pathological processes. These processes include cell differentiation, growth, angiogenesis, wound healing, and tumor development. FGFs exert their biological effects by binding to and activating type I transmembrane tyrosine kinase receptors. Upon ligand binding, these receptors dimerize and undergo autophosphorylation. Four distinct genes encode these FGF receptors. Alternative splicing of mRNA leads to multiple isoforms of FGFR-1, -2, and -3. A common splicing event in FGFR-1 and -2 generates receptors with all three immunoglobulin-like domains (alpha isoform) or only IgII and IgIII. Only the alpha isoform has been observed for FGFR-3 and FGFR-4. Further alternative splicing events in FGFR-1 to -3, affecting the C-terminal half of the IgIII domain encoded by two mutually exclusive exons, result in receptors with variant IgIII domains (IIIb and IIIc). A secreted FGF-binding protein, designated IIIa isoform, containing the N-terminal half of the IgIII domain and some intron sequences, has been identified for FGFR-1.

Produced in Sf9 insect cells using baculovirus expression system, FGFR2 is a single glycosylated polypeptide chain. This recombinant protein encompasses amino acids 22 to 289 and has a molecular weight of 56.8 kDa, including a 239 amino acid hIgG-His tag at the C-terminus. The purification process involves proprietary chromatographic techniques.

The FGFR2 protein solution is provided at a concentration of 0.5 mg/ml and is formulated in a buffer consisting of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

The purity of FGFR2 is greater than 95%, as assessed by SDS-PAGE analysis.

EC 2.7.10, FGFR-2, BFR-1, CD332, BBDS, CEK3, ECT1, TK14, TK25, CFD1, KSAM, JWS, Fibroblast Growth Factor Receptor 2, Keratinocyte Growth Factor Receptor, Bacteria-Expressed Kinase, EC 2.7.10.1, K-SAM, KGFR, BEK, Protein Tyrosine Kinase, Receptor Like 14, BEK Fibroblast Growth Factor Receptor, Craniofacial Dysostosis 1, Jackson-Weiss Syndrome, Pfeiffer Syndrome, Crouzon Syndrome, CD332 Antigen.

RPSFSLVEDT TLEPEDAISS GDDEDDTDGA EDFVSENSNN KRAPYWTNTE KMEKRLHAVP
AANTVKFRCP AGGNPMPTMR WLKNGKEFKQ EHRIGGYKVR NQHWSLIMES VVPSDKGNYT
CVVENEYGSI NHTYHLDVVE RSPHRPILQA GLPANASTVV GGDVEFVCKV YSDAQPHIQW
IKHVEKNGSK YGPDGLPYLK VLKHSGINSS NAEVLALFNV TEADAGEYIC KVSNYIGQAN
QSAWLTVLPK QQAPGREKEI TASPDYLELE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK
PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL
TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT
CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCSVMHEALHNHY TQKSLSLSPG KHHHHHH.