FGF 18 Human, His
Description
Product Specs
Fibroblast growth factor 18 (FGF18) belongs to the FGF family, a group of at least 23 growth factors. It possesses a central 120-amino acid FGF domain, contributing to a conserved tertiary structure. FGFs play roles during embryonic development and are found in specific adult tissues. FGF-18 is crucial for the development of long bones and the skull. Its signaling occurs through FGFR 1c, 2c, 3c, and 4.
To prepare a working stock solution, it is advised to add deionized water to achieve a concentration of approximately 0.5mg/ml. Allow the lyophilized pellet to dissolve completely. Note: FGF18 is not sterile. Ensure to filter the product using an appropriate sterile filter before using it in cell cultures.
Product Science Overview
Fibroblast Growth Factor-18 (FGF18) is a member of the fibroblast growth factor (FGF) family, which is known for its broad mitogenic and cell survival activities. These factors are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion .
FGF18 was first identified in 1998 when two newly-identified murine genes, Fgf17 and Fgf18, were described and confirmed as being closely related by sequence homology to Fgf8 . The FGF18 gene is located on chromosome 5 in humans and chromosome 11 in mice . The protein encoded by this gene is involved in several critical biological processes.
FGF18 consists of 207 amino acids and contains two potential N-linked glycosylation domains . It is structurally most homologous to FGF8 and FGF17 among the FGF family . The core 120 amino acid FGF domain allows for a common tertiary structure, which is crucial for its function .
FGF18 is a binding growth factor that signals via FGFR 1c, 2c, 3c, and 4 . It plays an indispensable role in the development of long bones and the calvaria (the upper part of the skull) . Additionally, FGF18 is expressed predominantly in the adult lungs and kidneys, as well as in several discrete regions during embryonic development .
Recombinant human FGF18 (rhFGF18) is produced using various expression systems, including mammalian cells and Escherichia coli . The His tag is a polyhistidine tag that is added to the protein to facilitate purification. This tag allows for the protein to be purified using metal affinity chromatography, which is a common technique in protein purification.
The recombinant form of FGF18 has been extensively used for fundamental research and clinical applications. Some of the key applications include:
- Chondrogenesis and Osteogenesis: FGF18 is crucial for the development and repair of cartilage and bone .
- Hair Growth: Studies have shown that rhFGF18 can effectively regulate hair growth .
- Neuroprotection: FGF18 has potential neuroprotective effects, making it a candidate for research in neurodegenerative diseases .
