Introduction
Rat and mouse FGF-9 share significant homology with human FGF-9. FGF-9 transcripts are present in brain and kidney tissue. As a member of the fibroblast growth factor (FGF) family, FGF-9 exhibits mitogenic and cell survival properties, contributing to various biological processes like embryonic development, cell growth, morphogenesis, tissue repair, and tumor progression. Initially identified as a secreted factor promoting glial cell growth in cultures, FGF-9 is primarily produced by neurons in the nervous system and potentially plays a role in glial cell development. Studies indicate that the expression of the mouse FGF-9 homolog relies on Sonic hedgehog (Shh) signaling. FGF-9 knockout mice exhibit a male-to-female sex reversal phenotype, suggesting its involvement in testicular embryogenesis. FGF-9 may contribute to glial cell growth and differentiation during development, gliosis in brain tissue repair after injury, neuronal cell differentiation and survival, and the stimulation of glial tumor growth.
Description
Recombinant Rat FGF9, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 207 amino acids with a molecular weight of 23.3 kDa. Purification of the recombinant mouse FGF-9 is achieved using proprietary chromatographic methods.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The FGF-9 was lyophilized from a sterile solution at a concentration of 1 mg/ml, containing 10 mM NaP (pH 7.5) and 75 mM Ammonium Sulfate.
Solubility
For reconstitution of the lyophilized Rat FGF-9, sterile 18 MΩ-cm H2O is recommended at a concentration not less than 100 µg/ml. This solution can be further diluted into other aqueous solutions as needed.
Stability
Lyophilized Rat Fibroblast Growth Factor-9, while stable at room temperature for 3 weeks, should ideally be stored desiccated at a temperature below -18 °C. After reconstitution, Rat Recombinant FGF9 can be stored at 4 °C for 2-7 days. For long-term storage, it is advisable to store at a temperature below -18 °C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 95.0% as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The ED50, determined by the dose-dependent proliferation of BAF3 cells expressing FGF receptors (measured via ³H-thymidine uptake), is < 0.5 ng/ml, corresponding to a specific activity of ≥ 2,000,000 Units/mg.
Synonyms
GAF (Glia-activating factor), HBGF-9, MGC119914, MGC119915, FGF-9.
Amino Acid Sequence
MPLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS.