Introduction
Human FGF-9, encoded by the FGF9 gene, is a 208-amino acid protein with a potential N-glycosylation site. It is synthesized as a glycosylated protein and efficiently secreted despite lacking a typical signal peptide. FGF-9 exhibits high homology across humans, rats, and mice. Transcripts of FGF-9 are found in brain and kidney tissues. As a member of the fibroblast growth factor (FGF) family, FGF-9 possesses mitogenic and cell survival activities, playing crucial roles in embryonic development, cell growth, morphogenesis, tissue repair, and tumor progression. Initially isolated as a secreted factor stimulating glial cell growth, FGF-9 is primarily produced by neurons in the nervous system and is implicated in glial cell development. Studies have shown that the expression of its mouse homolog depends on Sonic hedgehog (Shh) signaling. FGF-9 knockout mice exhibit a male-to-female sex reversal phenotype, suggesting its role in testicular embryogenesis. In summary, FGF-9 contributes to glial cell growth and differentiation during development, gliosis in brain tissue repair, neuronal cell differentiation and survival, and the stimulation of glial tumor growth.
Description
Recombinant Human Fibroblast Growth Factor-9, produced in E.coli, is a non-glycosylated polypeptide chain comprising 207 amino acids. With a molecular weight of 23.4 kDa, this single-chain protein is purified using proprietary chromatographic techniques.
Physical Appearance
Sterile, lyophilized powder with a white appearance.
Formulation
The protein is provided as a sterile, lyophilized powder. It is lyophilized from a solution containing 1 mg/ml of the protein in 1xPBS.
Solubility
To reconstitute the lyophilized Human Fibroblast Growth Factor-9, it is recommended to dissolve the powder in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. This solution can be further diluted in other aqueous solutions.
Stability
Lyophilized Human Fibroblast Growth Factor 9 remains stable at room temperature for up to 3 weeks. However, for long-term storage, it is recommended to store the lyophilized protein desiccated at a temperature below -18°C. After reconstitution, the FGF-9 solution can be stored at 4°C for 2-7 days. For extended storage, it should be kept at -18°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of this protein is determined to be greater than 95.0% using RP-HPLC and SDS-PAGE analysis.
Biological Activity
The biological activity of this product is determined by its ability to stimulate the proliferation of BAF3 cells expressing FGF receptors. The ED50, measured by 3H-thymidine uptake, is less than 0.5 ng/ml. This corresponds to a specific activity of 2,000,000 Units/mg.
Synonyms
GAF (Glia-activating factor), HBGF-9, MGC119914, MGC119915, FGF-9.
Amino Acid Sequence
APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV
TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA
VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN
LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELY
KDILSQS.
