FGF23 Human, Sf9
Sf9, Insect cells.
Fibroblast growth factor 23, FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor, HYPF.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
FGF23 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 236 amino acids (25-251a.a.) and having a molecular mass of 26.4kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).
FGF23 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Fibroblast growth factor 23 (FGF-23) belongs to the fibroblast growth factor (FGF) family, known for their roles in cell growth, survival, and various biological processes such as embryonic development, tissue repair, and morphogenesis. FGF-23 specifically acts to regulate phosphate metabolism by inhibiting phosphate transport in renal tubules. Mutations in the FGF23 gene are linked to autosomal dominant hypophosphatemic rickets (ADHR), a genetic disorder characterized by phosphate wasting. Additionally, elevated FGF23 expression is observed in oncogenic hypophosphatemic osteomalacia (OHO), a condition with similar characteristics arising from abnormal phosphate metabolism.
This product consists of the FGF23 protein produced in Sf9 insect cells. It is a single, glycosylated polypeptide chain with a molecular weight of 26.4 kDa. The protein sequence encompasses amino acids 25 to 251a.a. and includes a 9 amino acid His tag at the C-terminus. The protein appears as a band around 13.5-18kDa on SDS-PAGE due to glycosylation. Purification is achieved through proprietary chromatographic techniques.
The product appears as a clear, colorless solution that has been sterilized by filtration.
The FGF23 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution is composed of Phosphate Buffered Saline (pH 7.4) with 2mM DTT, 1mM EDTA, and 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. To maintain product integrity, avoid repeated freeze-thaw cycles.
The purity of the FGF23 protein in this product is greater than 90% as determined by SDS-PAGE analysis.
Fibroblast growth factor 23, FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor, HYPF.
Sf9, Insect cells.
ADPYPNASPL LGSSWGGLIH LYTATARNSY HLQIHKNGHV DGAPHQTIYS ALMIRSEDAG
FVVITGVMSR RYLCMDFRGN IFGSHYFDPE NCRFQHQTLE NGYDVYHSPQ YHFLVSLGRA
KRAFLPGMNP PPYSQFLSRR NEIPLIHFNT PIPRRHTRSA EDDSERDPLN VLKPRARMTP
APASCSQELP SAEDNSPMAS DPLGVVRGGR VNTHAGGTGP EGCRPFAKFI HHHHHH.
Product Science Overview
FGF-23 produced in Sf9 insect cells is a single, glycosylated polypeptide chain containing 236 amino acids (25-251a.a.) and has a molecular mass of approximately 26.4 kDa . The protein is expressed with a 9 amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .
FGF-23 plays a crucial role in regulating phosphate homeostasis and vitamin D metabolism. It decreases the reabsorption of phosphate in the kidneys, thereby increasing phosphate excretion . Additionally, FGF-23 suppresses the biosynthesis of 1,25-dihydroxyvitamin D3, a hormone involved in calcium and phosphate metabolism .
Mutations in the FGF23 gene are associated with several disorders:
- Autosomal Dominant Hypophosphatemic Rickets (ADHR): An inherited disorder characterized by phosphate wasting .
- Oncogenic Hypophosphatemic Osteomalacia (OHO): A disease caused by abnormal phosphate metabolism .
- Familial Tumoral Calcinosis with Hyperphosphatemia: A condition resulting from FGF-23 mutations .
Lyophilized FGF-23 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use and below -18°C for future use. It is recommended to add a carrier protein (0.1% HSA or BSA) to prevent freeze-thaw cycles .
