FGB Human

Fibrinogen Beta Chain Human Recombinant
Cat. No.
BT8762
Source
Escherichia Coli.
Synonyms
Fibrinogen beta chain, Fibrinopeptide B, Fibrinogen, B Beta Polypeptide, Epididymis Secretory Sperm Binding Protein Li 78p, HEL-S-78p.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FGB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 351 amino acids (164-491a.a) and having a molecular mass of 40kDa. 
FGB is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Fibrinogen Beta Chain 9 (FGB) is one of the three components of fibrinogen, a glycoprotein found in blood. It is composed of three pairs of polypeptide chains, each with a unique structure. Upon vascular injury, the enzyme thrombin acts on fibrinogen, cleaving it to form fibrin, a key protein involved in blood clot formation.
Description
Recombinant Human FGB, produced in E. coli, is a single polypeptide chain devoid of glycosylation. It encompasses amino acids 164 to 491, resulting in a protein with a molecular weight of 40 kDa. The N-terminus of the FGB protein is fused with a 23 amino acid His-tag. The protein undergoes purification using proprietary chromatographic methods.
Physical Appearance
A clear, sterile solution without any color.
Formulation
The FGB protein is provided in a solution with a concentration of 1 mg/ml. The solution contains 20 mM Tris-HCl buffer at a pH of 8.0 and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), keep the vial at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage to enhance protein stability. Avoid repeated freezing and thawing cycles.
Purity
The purity of the FGB protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
Fibrinogen beta chain, Fibrinopeptide B, Fibrinogen, B Beta Polypeptide, Epididymis Secretory Sperm Binding Protein Li 78p, HEL-S-78p.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSDNENVVN EYSSELEKHQ LYIDETVNSN IPTNLRVLRS ILENLRSKIQ KLESDVSAQM EYCRTPCTVS CNIPVVSGKE CEEIIRKGGE TSEMYLIQPD SSVKPYRVYC DMNTENGGWT VIQNRQDGSV DFGRKWDPYK QGFGNVATNT DGKNYCGLPG EYWLGNDKIS QLTRMGPTEL LIEMEDWKGD KVKAHYGGFT VQNEANKYQI SVNKYRGTAG NALMDGASQL MGENRTMTIH NGMFFSTYDR DNDGWLTSDP RKQCSKEDGG GWWYNRCHAA NPNGRYYWGG QYTWDMAKHG TDDGVVWMNW KGSWYSMRKM SMKIRPFFPQ Q.

Product Science Overview

Structure and Function

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It consists of three pairs of polypeptide chains: Aα, Bβ, and γ. The beta chain (Bβ) is one of these critical components. Upon vascular injury, thrombin cleaves fibrinogen to form fibrin monomers, which then polymerize to form an insoluble fibrin matrix . This matrix is essential for hemostasis, as it stabilizes blood clots and aids in wound healing .

Genetic Information

The FGB gene is located on chromosome 4 in humans . Mutations in this gene can lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia, and thrombotic tendencies . These conditions can affect the blood’s ability to clot properly, leading to either excessive bleeding or an increased risk of thrombosis.

Recombinant Fibrinogen Beta Chain

Recombinant fibrinogen beta chain is produced using recombinant DNA technology, which involves inserting the FGB gene into a host cell to produce the protein. This technology allows for the production of large quantities of the protein for research and therapeutic purposes. Recombinant fibrinogen beta chain is used in various applications, including studying blood clotting mechanisms, developing new treatments for bleeding disorders, and creating synthetic blood products .

Clinical Significance

Fibrinogen and its cleavage products play several roles beyond clot formation. They regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and act as mitogens for various cell types . These functions make fibrinogen a critical player in wound healing and tissue repair. Additionally, abnormalities in fibrinogen levels or function can serve as biomarkers for various diseases, including cardiovascular diseases and certain cancers .

Research and Therapeutic Applications

Research on fibrinogen beta chain has led to significant advancements in understanding blood coagulation and related disorders. Recombinant fibrinogen beta chain is used in developing assays to measure fibrinogen levels and activity in clinical samples. It is also being explored as a potential therapeutic agent for treating bleeding disorders and improving wound healing .

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