FCGR3B Human
Fc Fragment Of IgG Receptor IIIb, Fc Fragment Of IgG, Low Affinity IIIb, Receptor (CD16b), Fc Gamma Receptor IIIb, IgG Fc Receptor III-1, Fc-Gamma RIII-Beta, Fc-Gamma RIIIb, FCRIIIb, FCR-10, FCRIII, FCGR3, CD16b, FCG3, Fc Fragment Of IgG, Low Affinity IIIb, Receptor For (CD16), Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B, Fc-Gamma Receptor IIIb (CD 16), Fc-Gamma RIII, CD16b Antigen, IGFR3, CD16.
Greater than 90.0% as determined by SDS-PAGE.
Description
FCGR3B produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 188 amino acids (21-200a.a.) and having a molecular mass of 21.4kDa. (Molecular size on SDS-PAGE will appear at approximately 28-40kDa).
FCGR3B is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Fc Fragment Of IgG Receptor IIIb, Fc Fragment Of IgG, Low Affinity IIIb, Receptor (CD16b), Fc Gamma Receptor IIIb, IgG Fc Receptor III-1, Fc-Gamma RIII-Beta, Fc-Gamma RIIIb, FCRIIIb, FCR-10, FCRIII, FCGR3, CD16b, FCG3, Fc Fragment Of IgG, Low Affinity IIIb, Receptor For (CD16), Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B, Fc-Gamma Receptor IIIb (CD 16), Fc-Gamma RIII, CD16b Antigen, IGFR3, CD16.
EDLPKAVVFL EPQWYSVLEK DSVTLKCQGA YSPEDNSTQW FHNENLISSQ ASSYFIDAAT VNDSGEYRCQ TNLSTLSDPV QLEVHIGWLL LQAPRWVFKE EDPIHLRCHS WKNTALHKVT YLQNGKDRKY FHHNSDFHIP KATLKDSGSY FCRGLVGSKN VSSETVNITI TQGLAVSTIS VEHHHHHH.
Product Science Overview
CD16b is primarily expressed on neutrophils and has numerous polymorphic variants. The most relevant variants are associated with human neutrophil antigens (HNA) -1a, -1b, and -1c . These variants differ in their amino acid sequences, which can affect their function and interaction with antibodies. For example, HNA-1a and HNA-1b differ in four amino acids at positions 36, 65, 82, and 106, while HNA-1c has an additional mutation at position 78 .
CD16b is involved in several critical immune functions:
- Phagocytosis: It aids in the engulfment and digestion of pathogens by neutrophils.
- Clearance of Immune Complexes: CD16b helps in the removal of antibody-bound antigens from the bloodstream.
- Antibody-Dependent Cellular Cytotoxicity (ADCC): It plays a role in the destruction of antibody-coated target cells by immune cells .
Recombinant human CD16b is produced using DNA sequences encoding the protein, which are expressed in host cells such as CHO (Chinese Hamster Ovary) cells. The recombinant protein typically includes a polyhistidine tag for purification purposes . The recombinant CD16b comprises 195 amino acids with a predicted molecular mass of 22.2 kDa, but due to glycosylation, it migrates as an approximately 38-43 kDa band in SDS-PAGE under reducing conditions .
CD16b’s polymorphic variants can lead to the production of alloantibodies, which are responsible for conditions such as neonatal alloimmune neutropenia (NIN), autoimmune neutropenia of infancy (AIN), and transfusion-related acute lung injury (TRALI) . Understanding the structure and function of CD16b is crucial for developing therapeutic strategies to manage these conditions.
