FAS Antibody
Description
Product Specs
Product Science Overview
The FAS Blocking/Activating Antibody, also known as CD95, is a monoclonal antibody derived from mice and is specifically designed to target human FAS (CD95) proteins. This antibody plays a crucial role in the study of apoptosis, a form of programmed cell death, by either blocking or activating the FAS receptor.
FAS, also known as CD95 or APO-1, is a cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. It is widely expressed on the surface of various cell types, including both normal and transformed cells. The primary function of FAS is to mediate apoptosis when it binds to its ligand, FASL (CD95L/Apo-1L). This interaction triggers a cascade of intracellular signaling events leading to cell death .
The FAS Blocking/Activating Antibody can function in two distinct ways:
- Blocking: When in soluble form, the antibody can block the interaction between FAS and its ligand, thereby inhibiting the apoptotic signaling pathway. This is particularly useful in research focused on preventing cell death in various diseases.
- Activating: When immobilized, the antibody can mimic the action of FASL by binding to the FAS receptor and inducing apoptosis. This property is valuable in studies aimed at understanding the mechanisms of cell death and in developing therapies for conditions where inducing apoptosis is beneficial .
The FAS Blocking/Activating Antibody is utilized in a variety of research applications, including:
- Direct ELISA: Used to detect and quantify the presence of FAS in samples.
- Flow Cytometry: Employed to analyze the expression of FAS on the surface of cells.
- Immunohistochemistry (IHC): Applied to visualize the distribution and localization of FAS in tissue sections.
- Functional Assays: Used to study the effects of blocking or activating FAS-mediated apoptosis in cell cultures .
The antibody is typically shipped in a lyophilized form at ambient temperature. For long-term storage, it should be kept at -20°C. After reconstitution with water, it is recommended to aliquot and store the antibody at -20°C if not used within a month. Proper storage ensures the stability and efficacy of the antibody for up to 12 months .
