FABP6 Human, His
Fatty Acid Binding Protein 6 Human Recombinant, His Tag
Cat. No.
BT7305
Source
Escherichia Coli.
Synonyms
I-BABP, ILBP, I-15P, I-BAP, ILBP3, ILLBP, I-BABP, I-BALB, FABP-6, Gastrotropin, Ileal lipid-binding protein, Intestinal 15 kDa protein, Intestinal bile acid-binding protein, Fatty acid-binding protein 6, FABP6.
Appearance
Sterile Filtered colorless liquid formulation.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
FABP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 128 amino acids and having a molecular mass of 18 kDa. FABP6 is fused to His tag at N-terminus and purified by standard chromatography techniques.
Product Specs
Introduction
FABP6, also known as ileal fatty acid binding protein, belongs to a small family of highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein plays a crucial role in binding bile acid and is involved in fatty acid uptake, transport, and metabolism. Additionally, FABP6 stimulates gastric acid and pepsinogen secretion and exhibits binding affinity for bile salts and bilirubins. Its expression is restricted to the ileum in the small intestine, where it participates in the enterohepatic circulation of bile acids. Two transcript variants are generated by alternate transcription promoters, encoding proteins of 128 and 177 amino acid residues. Human FABP6 isoform 2 consists of 128 amino acids and is acetylated at Ala2. This protein binds both fatty acids and bile acids, playing a direct role in fatty acid transport and metabolism.
Description
Recombinant Human FABP6, expressed in E. coli, is a single, non-glycosylated polypeptide chain with a molecular weight of 18 kDa. This protein comprises 128 amino acids and has a His tag fused at its N-terminus. It is purified using standard chromatography techniques.
Physical Appearance
Sterile, colorless liquid.
Formulation
FABP6 His-Tag is supplied in a buffer consisting of 20mM Tris-HCl (pH 8), 0.5mM DTT, and 50% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
I-BABP, ILBP, I-15P, I-BAP, ILBP3, ILLBP, I-BABP, I-BALB, FABP-6, Gastrotropin, Ileal lipid-binding protein, Intestinal 15 kDa protein, Intestinal bile acid-binding protein, Fatty acid-binding protein 6, FABP6.
Source
Escherichia Coli.
Product Science Overview
Introduction
Fatty Acid Binding Protein 6 (FABP6), also known as ileal bile acid-binding protein (I-BABP), is a member of the fatty acid-binding protein family. These proteins are small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands . FABP6 is specifically expressed in the ileum, the final section of the small intestine, and plays a crucial role in the uptake, transport, and metabolism of fatty acids and bile acids .
Structure and Characteristics
The human recombinant FABP6 with an N-terminal His tag is produced in Escherichia coli (E. coli). This recombinant protein consists of 128 amino acids and has a molecular weight of approximately 18 kDa . The His tag facilitates the purification of the protein using affinity chromatography techniques .
Function
FABP6 is involved in several critical biological processes:
- Fatty Acid Uptake and Transport: FABP6 binds long-chain fatty acids, facilitating their transport within the cell .
- Bile Acid Binding: Unlike other fatty acid-binding proteins, FABP6 also binds bile acids, which are essential for the digestion and absorption of dietary fats .
- Metabolism: By binding fatty acids and bile acids, FABP6 plays a role in their metabolism, influencing various metabolic pathways .
Applications
Recombinant FABP6 is used in various research applications, including:
- Immunological Assays: As a positive control in Western blotting and other immunological assays .
- Biochemical Studies: To study the binding properties and interactions of fatty acids and bile acids with FABP6 .
- Drug Development: As a target for developing drugs that modulate fatty acid and bile acid metabolism .
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