FABP6 Human

Fatty Acid Binding Protein-6 Human Recombinant
Cat. No.
BT7215
Source
Escherichia Coli.
Synonyms
I-BABP, ILBP, I-15P, I-BAP, ILBP3, ILLBP, I-BABP, I-BALB, FABP-6, Gastrotropin, Ileal lipid-binding protein, Intestinal 15 kDa protein, Intestinal bile acid-binding protein, Fatty acid-binding protein 6, FABP6.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

FABP6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 128 amino acids and having a molecular mass of 14 kDa.

Product Specs

Introduction
FABP6, also known as ileal fatty acid binding protein, belongs to a small family of highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein plays a crucial role in the uptake, transport, and metabolism of fatty acids. Additionally, FABP6 binds bile acids and is involved in their enterohepatic circulation in the ileum. It is also believed to stimulate gastric acid and pepsinogen secretion. There are two transcript variants of FABP6, resulting from alternate transcription promoters, which encode a 128 amino acid and a 177 amino acid residue protein, respectively. The human FABP6 isoform 2 comprises 128 amino acid residues and undergoes acetylation at Ala2. This protein demonstrates the ability to bind both fatty acids and bile acids, indicating its direct involvement in fatty acid transport and metabolism.
Description
Recombinant human FABP6, expressed in E. coli, is a single, non-glycosylated polypeptide chain consisting of 128 amino acids with a molecular weight of 14 kDa.
Physical Appearance
The product is a clear and colorless sterile filtered solution.
Formulation
The FABP6 protein is supplied in a solution containing 1xPBS (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), store the protein at 4°C. For extended storage, freeze the protein at -20°C. To ensure long-term stability during frozen storage, it is recommended to add a carrier protein such as HSA or BSA at a concentration of 0.1%. Avoid repeated freeze-thaw cycles.
Purity
The purity of FABP6 is greater than 95.0% as determined by: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) (b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
Synonyms
I-BABP, ILBP, I-15P, I-BAP, ILBP3, ILLBP, I-BABP, I-BALB, FABP-6, Gastrotropin, Ileal lipid-binding protein, Intestinal 15 kDa protein, Intestinal bile acid-binding protein, Fatty acid-binding protein 6, FABP6.
Source
Escherichia Coli.
Amino Acid Sequence
MAFTGKFEME SEKNYDEFMK LLGISSDVIE KAHNFKIVTE VQQDGQDFTW SQHYYGGHTM TNKFTVGKES NIQTMGGKTF KATVQMEGGK LVVNFPNYHQ TSEIVGDKLV EVSTIGGVTY ERVSKRLA.

Product Science Overview

Gene and Protein Structure

The FABP6 gene encodes the ileal fatty acid-binding protein. It is located on chromosome 5 in humans and has several aliases, including I-15P, I-BABP, I-BALB, I-BAP, ILBP, ILBP3, and ILLBP . The protein itself is approximately 15 kDa in size and is primarily expressed in the ileum, a part of the small intestine .

Function and Role

FABP6 plays a crucial role in the enterohepatic circulation of bile acids. It binds to bile acids and is involved in their efficient transport from the apical to the basolateral side of ileal enterocytes . This protein is essential for the uptake, transport, and metabolism of fatty acids and bile acids . In vitro studies have shown that FABP6 binds to bile acids in the order: deoxycholic acid > cholic acid > chenodeoxycholic acid, with affinities modified by conjugation (taurine-conjugated > glycine-conjugated > unconjugated bile acids) .

Clinical Significance

FABP6 has been associated with various diseases, including colorectal adenocarcinoma and mucinous stomach adenocarcinoma . Its role in bile acid metabolism makes it a potential target for therapeutic interventions in diseases related to bile acid dysregulation.

Recombinant FABP6

Human recombinant FABP6 is produced using recombinant DNA technology, which involves inserting the FABP6 gene into an expression system to produce the protein in large quantities. This recombinant protein is used in various research applications to study its function, interactions, and potential therapeutic uses .

Research and Applications

Research on FABP6 includes studying its role in fatty acid uptake, transport, and metabolism. It is also investigated for its involvement in bile acid-induced apoptosis in colon cancer cells . The recombinant form of FABP6 is valuable for these studies as it allows for controlled experiments and detailed analysis of the protein’s properties and functions.

In summary, Fatty Acid Binding Protein-6 (Human Recombinant) is a vital protein involved in the metabolism and transport of fatty acids and bile acids. Its role in various physiological processes and diseases makes it an important subject of research in biochemistry and molecular biology.

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Cat. No.
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Source
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