EXOSC8 Human
Exosome Component 8 Human Recombinant
Cat. No.
BT5887
Source
Escherichia Coli.
Synonyms
Exosome Component 8, EXOSC8, OIP2, RRP43, CBP-Interacting Protein 3, Opa Interacting Protein 2, Opa-Interacting Protein 2, Ribosomal RNA-Processing Protein 43, OIP-2, p9, CIP3, EAP2, Rrp43p, bA421P11.3, Exosome Complex Component RRP43, Exosome Complex Exonuclease RRP43.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EXOSC8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (1-276) and having a molecular mass of 32.4 kDa.
EXOSC8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
EXOSC8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
EXOSC8, also known as Exosome component 8 and transcription factor 4, is a vital component of the exosome complex. This complex plays a crucial role in cytoplasmic RNA degradation, including general mRNA turnover and targeted degradation of mRNAs with AU-rich elements (AREs) in their 3' untranslated regions. EXOSC8 is specifically involved in the degradation of histone mRNA and binds to ARE-containing RNAs. Essentially, EXOSC8 acts as a 3'-5' exoribonuclease, contributing to the RNA surveillance pathways by preventing the translation of faulty mRNAs.
Description
Recombinant EXOSC8 Human, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 299 amino acids, with a sequence spanning from amino acid 1 to 276, and has a molecular weight of 32.4 kDa. For purification purposes, a 23 amino acid His-tag is fused to the N-terminus, and the protein is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
EXOSC8 is provided as a solution at a concentration of 0.25 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 50% glycerol, 0.2M NaCl, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain product integrity.
Purity
Purity of EXOSC8 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Exosome Component 8, EXOSC8, OIP2, RRP43, CBP-Interacting Protein 3, Opa Interacting Protein 2, Opa-Interacting Protein 2, Ribosomal RNA-Processing Protein 43, OIP-2, p9, CIP3, EAP2, Rrp43p, bA421P11.3, Exosome Complex Component RRP43, Exosome Complex Exonuclease RRP43.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAGFKT VEPLEYYRRF LKENCRPDGR ELGEFRTTTV NIGSISTADG SALVKLGNTT VICGVKAEFA APSTDAPDKG YVVPNVDLPP LCSSRFRSGP PGEEAQVASQ FIADVIENSQ IIQKEDLCIS PGKLVWVLYC DLICLDYDGN ILDACTFALL AALKNVQLPE VTINEETALA EVNLKKKSYL NIRTHPVATS FAVFDDTLLI VDPTGEEEHL ATGTLTIVMD EEGKLCCLHK PGGSGLTGAK LQDCMSRAVT RHKEVKKLMD EVIKSMKPK.
Product Science Overview
Structure and Composition
Function
EXOSC8 plays a significant role in RNA processing and degradation. It specifically interacts with mRNAs containing AU-rich elements, which are sequences that signal the RNA molecule for degradation . This interaction is vital for maintaining the proper levels of various RNA species within the cell, ensuring that defective or excess RNAs are efficiently degraded.
Applications
Storage and Handling
Summary
In summary, Exosome Component 8 is a vital part of the RNA degradation machinery in cells. Its recombinant form, produced in E. coli, is a valuable tool for researchers studying RNA processing and the exosome complex’s function.
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