EXOSC7 Human
Exosome Component 7 Human Recombinant
Cat. No.
BT5835
Source
Escherichia Coli.
Synonyms
Exosome Component 7, RRP42, Ribosomal RNA-Processing Protein 42, P8, Exosome Complex Exonuclease RRP42, Exosome Complex Component RRP42, KIAA0116, HRrp42p, EAP1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EXOSC7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 314 amino acids (1-291 a.a.) and having a molecular mass of 34.2kDa.
EXOSC7 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
EXOSC7, a part of the RNA exosome complex, belongs to the RNase PH family. It participates in various pathways, including the transfer to a nascent protein, the Unfolded Protein Response, and the Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO). EXOSC9 is a significant paralog of this gene.
Description
Recombinant Human EXOSC7, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 314 amino acids (1-291 a.a.) with a molecular weight of 34.2 kDa. It consists of a 23 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The EXOSC7 protein solution has a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
The product should be stored at 4°C for 2-4 weeks if the entire vial will be used. For longer storage, it is recommended to store the product frozen at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is recommended. It is important to avoid subjecting the product to multiple freeze-thaw cycles.
Purity
The purity of the product is greater than 85.0% as determined by SDS-PAGE analysis.
Synonyms
Exosome Component 7, RRP42, Ribosomal RNA-Processing Protein 42, P8, Exosome Complex Exonuclease RRP42, Exosome Complex Component RRP42, KIAA0116, HRrp42p, EAP1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASVTLS EAEKVYIVHG VQEDLRVDGR GCEDYRCVEV ETDVVSNTSG SARVKLGHTD ILVGVKAEMG TPKLEKPNEG YLEFFVDCSA SATPEFEGRG GDDLGTEIAN TLYRIFNNKS SVDLKTLCIS PREHCWVLYV DVLLLECGGN LFDAISIAVK AALFNTRIPR VRVLEDEEGS KDIELSDDPY DCIRLSVENV PCIVTLCKIG YRHVVDATLQ EEACSLASLL VSVTSKGVVT CMRKVGKGSL DPESIFEMME TGKRVGKVLH ASLQSVVHKE ESLGPKRQKV GFLG
Product Science Overview
Structure and Function
EXOSC7 is a non-catalytic component of the RNA exosome complex, which exhibits 3’->5’ exoribonuclease activity. This activity is essential for the degradation of inherently unstable mRNAs containing AU-rich elements (AREs) within their 3’-untranslated regions . The RNA exosome complex is involved in multiple cellular processes, including:
- Maturation of Stable RNA Species: In the nucleus, the RNA exosome complex aids in the proper maturation of stable RNA species such as rRNA, snRNA, and snoRNA .
- Elimination of RNA Processing By-products: It helps in the elimination of RNA processing by-products and non-coding ‘pervasive’ transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs) .
- mRNA Turnover: In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs .
- RNA Surveillance Pathways: It prevents the translation of aberrant mRNAs through RNA surveillance pathways .
Recombinant Human Exosome Component 7
Recombinant Human Exosome Component 7 is produced using E. coli expression systems and is typically tagged with a His-tag for purification purposes . The recombinant protein corresponds to the amino acids 1-291 of the human EXOSC7 protein . It is used extensively in research to study the function and structure of the RNA exosome complex.
Applications in Research
The recombinant form of EXOSC7 is valuable in various research applications, including:
- Structural Studies: Understanding the structure of the RNA exosome complex and its components.
- Functional Assays: Investigating the role of EXOSC7 in RNA processing and degradation.
- Disease Research: Studying the implications of EXOSC7 in diseases associated with RNA metabolism, such as Pontocerebellar Hypoplasia, Type 1A .
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