EXOSC5 Human
Exosome Component 5 Human Recombinant
Cat. No.
BT5776
Source
E.coli.
Synonyms
Exosome component 5, Chronic myelogenous leukemia tumor antigen 28, Ribosomal RNA-processing protein 46, exosome complex exonuclease RRP46, RRP41B, hRrp46p, CML28, p12B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
EXOSC5 Human Recombinant produced in E. coli is a single polypeptide chain containing 256 amino acids (1-235) and having a molecular mass of 27.5kDa.
EXOSC5 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
EXOSC5 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
EXOSC5, a member of the RNase PH family, interacts with Exosome component 8 and Exosome component 1. This non-catalytic component of the RNA exosome complex exhibits 3'->5' exoribonuclease activity and participates in numerous cellular RNA processing and degradation events. EXOSC5 expression is observed in a wide array of hematopoietic and epithelial tumor cell lines, contrasting its absence in normal hematopoietic tissues or other standard tissues, with the exception of the testis.
Description
Recombinant human EXOSC5, produced in E. coli, is a single polypeptide chain comprising 256 amino acids (1-235) with a molecular weight of 27.5 kDa. The protein includes a 21 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The EXOSC5 solution (0.5 mg/mL) is supplied in PBS buffer (pH 7.4) containing 200 mM NaCl, 2 mM DTT, and 30% glycerol.
Stability
For short-term storage (2-4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeding 90% as determined by SDS-PAGE analysis.
Synonyms
Exosome component 5, Chronic myelogenous leukemia tumor antigen 28, Ribosomal RNA-processing protein 46, exosome complex exonuclease RRP46, RRP41B, hRrp46p, CML28, p12B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MMEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS
Product Science Overview
Structure and Production
The human recombinant EXOSC5 is typically produced in Escherichia coli (E. coli) as a single polypeptide chain containing 256 amino acids, with a molecular mass of approximately 27.5 kDa . It is often fused to a 21 amino acid His-tag at the N-terminus to facilitate purification through chromatographic techniques .
Function
EXOSC5 is a part of the RNA exosome complex, which has 3’ to 5’ exoribonuclease activity. This complex is involved in the processing of structured RNAs and the degradation of aberrant non-coding RNAs . The RNA exosome complex plays a crucial role in maintaining RNA homeostasis by participating in various cellular RNA processing and degradation events .
Applications
Importance in Research
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