EXOSC1 Human

EXOSC1 is a single, non-glycosylated polypeptide chain consisting of 218 amino acids, with a molecular mass of approximately 23.8 kDa . The recombinant form of this protein is typically produced in Escherichia coli (E. coli) and includes a 23 amino acid His-tag at the N-terminus to facilitate purification .

The exosome complex, where EXOSC1 is a core component, is essential for the degradation of various RNA species, including messenger RNA (mRNA), ribosomal RNA (rRNA), and small nuclear RNA (snRNA). This complex is involved in both the cytoplasmic and nuclear RNA decay pathways, ensuring the proper regulation of RNA levels within the cell .

EXOSC1, specifically, is known to be a marker for human myeloid cells. Studies have shown that it is expressed during the early phases of myeloid differentiation, indicating its role in the development and function of these cells .

Recombinant EXOSC1 is widely used in research to study the mechanisms of RNA processing and degradation. Its availability in a purified form allows researchers to conduct detailed biochemical and structural analyses, contributing to a deeper understanding of the exosome complex’s function and regulation .

For optimal stability, EXOSC1 should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to keep the protein frozen at -20°C, with the addition of a carrier protein such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .