Exodus-2 Mouse, Sf9
Sf9, Baculovirus cells.
Ccl21a, C-C motif chemokine 21a, 6Ckine, Beta-chemokine exodus-2, Small-inducible cytokine A21a,Thymus-derived chemotactic agent 4, TCA4, Ccl21a, Scya21, Scya21a, 6CKBAC2, 6Ckine, ALP,AW987545, CKb9, plt, Scya21b, SLC.
Greater than 90.0% as determined by SDS-PAGE.
Description
Exodus-2 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 119 amino acids (24-133aa) and having a molecular mass of 13.1kDa.
Exodus-2 is fused to a 9 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Ccl21a, C-C motif chemokine 21a, 6Ckine, Beta-chemokine exodus-2, Small-inducible cytokine A21a,Thymus-derived chemotactic agent 4, TCA4, Ccl21a, Scya21, Scya21a, 6CKBAC2, 6Ckine, ALP,AW987545, CKb9, plt, Scya21b, SLC.
Sf9, Baculovirus cells.
ADPSDGGGQD CCLKYSQKKI PYSIVRGYRK QEPSLGCPIP AILFSPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS PGCRKNRGTS KSGKKGKGSK GCKRTEQTQP SRGHHHHHH
Product Science Overview
Exodus-2, also known as Chemokine (C-C motif) ligand 21 (CCL21), is a small cytokine belonging to the CC chemokine family. This chemokine is also referred to as 6Ckine due to its six conserved cysteine residues, which is a unique feature compared to the typical four cysteines found in other chemokines. Exodus-2 plays a crucial role in the immune system by acting as a chemoattractant for lymphocytes, guiding them to secondary lymphoid tissues. The gene encoding CCL21 is located on human chromosome 9.
Exodus-2 (CCL21) can be produced recombinantly in various host systems, including Escherichia coli and Sf9 Baculovirus cells. The recombinant production in Sf9 Baculovirus cells involves the following steps:
- Gene Cloning: The gene encoding mouse CCL21 is cloned into a baculovirus expression vector.
- Transfection: The recombinant baculovirus vector is used to transfect Sf9 insect cells.
- Expression: The transfected Sf9 cells express the CCL21 protein, which is secreted into the culture medium.
- Purification: The recombinant CCL21 protein is purified using proprietary chromatographic techniques. The resulting protein is a single, glycosylated polypeptide chain containing 119 amino acids and has a molecular mass of 13.1 kDa .
Exodus-2 (CCL21) binds to the chemokine receptor CCR7, which is located on the surface of various immune cells. This binding triggers a cascade of intracellular signaling events that lead to the directed migration of lymphocytes to secondary lymphoid tissues. The interaction between CCL21 and CCR7 is essential for the proper functioning of the immune system, particularly in the context of immune surveillance and the initiation of immune responses.
The recombinant Exodus-2 (CCL21) produced in Sf9 Baculovirus cells is characterized by its high purity and stability. It is typically formulated in a phosphate-buffered saline solution with 10% glycerol to maintain its stability during storage. The protein is stored at 4°C for short-term use and at -20°C for long-term storage to prevent degradation .
