Exodus 2 Mouse
Exodus-2 Mouse Recombinant (CCL21)
Cat. No.
BT12147
Source
Escherichia Coli.
Synonyms
Small inducible cytokine A21, CCL21, Beta chemokine exodus-2, 6Ckine, Secondary lymphoid-tissue chemokine, SLC, chemokine (C-C motif) ligand 21, ECL, CKb9, TCA4, SCYA21, MGC34555.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by SDS-PAGE & RP-HPLC.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
Exodus-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 110 amino acids and having a molecular mass of 12kDa.
The CCL21 is purified by proprietary chromatographic techniques.
The CCL21 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Chemokine (C-C motif) ligand 21 (CCL21), also called 6Ckine, exodus-2, and secondary lymphoid-tissue chemokine (SLC), is a small cytokine in the CC chemokine family. Unlike other CC chemokines, CCL21 primarily attracts lymphocytes and is mainly found in the lymph nodes. The gene responsible for CCL21 is found on chromosome 9 in humans. CCL21 interacts with a cell surface receptor called CCR7 to exert its effects.
Description
Recombinant Exodus-2 from mice, produced in E. coli, is a single, non-glycosylated polypeptide chain. It contains 110 amino acids, has a molecular weight of 12kDa, and is purified using proprietary chromatographic techniques.
Physical Appearance
White, sterile-filtered powder, freeze-dried.
Formulation
Lyophilized from a 1 mg/ml solution in 20mM Phosphate buffer with 0.15M NaCl, at pH 7.4.
Solubility
Reconstitute the lyophilized Exodus-2 in sterile 18 MΩ-cm H2O to a concentration of at least 100 µg/ml. Further dilutions can be made in other aqueous solutions.
Stability
Lyophilized Exodus-2 is stable at room temperature for up to 3 weeks. For long-term storage, store desiccated below -18°C. After reconstitution, store CCL21 at 4°C for 2-7 days. For future use, store below -18°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0%, as determined by SDS-PAGE and RP-HPLC analysis.
Biological Activity
The biological activity is assessed by the ability to attract murine T cells within a concentration range of 10-100 ng/ml. This corresponds to a specific activity of 10,000-100,000 IU/mg.
Synonyms
Small inducible cytokine A21, CCL21, Beta chemokine exodus-2, 6Ckine, Secondary lymphoid-tissue chemokine, SLC, chemokine (C-C motif) ligand 21, ECL, CKb9, TCA4, SCYA21, MGC34555.
Source
Escherichia Coli.
Amino Acid Sequence
SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE
LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK
RTEQTQPSRG.
Product Science Overview
Discovery and Structure
Exodus-2 was discovered independently by three groups from the EST (Expressed Sequence Tag) database . It shows 21-33% identity to other CC chemokines . The protein contains the four conserved cysteines characteristic of β chemokines, plus two additional cysteines in its unusually long carboxyl terminal domain .
Biological Activity
Exodus-2 is known for its ability to chemoattract T and B lymphocytes and inhibit hematopoiesis . It mediates the homing of lymphocytes to the lymphatic system, which is essential for the immune response . The biological activity of Exodus-2 has been determined through chemotaxis bioassays using murine T-lymphocytes, showing activity in a concentration range of 10.0-100.0 ng/ml .
Recombinant Production
Applications
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
