ETS2 Human

V-Ets Avian Erythroblastosis Virus E26 Oncogene 2 Human Recombinant
Cat. No.
BT8484
Source
Escherichia Coli.
Synonyms
Protein C-ets-2, ETS2, V-Ets Avian Erythroblastosis Virus E26 Oncogene 2, V-ets erythroblastosis virus E26 oncogene homolog 2, ETS2IT1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ETS2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 489 amino acids (1-469 a.a) and having a molecular mass of 55.1kDa.
ETS2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The V-Ets Avian Erythroblastosis Virus E26 Oncogene 2 (ETS2), a transcription factor, plays a crucial role in regulating gene expression related to developmental processes and programmed cell death (apoptosis). This protein is also recognized as a proto-oncogene, indicating its potential involvement in cancer development. Notably, ETS2 participates in the intricate regulation of telomerase, an enzyme crucial for maintaining the protective ends of chromosomes. Interestingly, a non-functional copy of the ETS2 gene, termed a pseudogene, is located on the X chromosome.
Description
Recombinant human ETS2, produced in E. coli bacteria, is a single polypeptide chain that lacks glycosylation modifications. It comprises 489 amino acids, spanning from position 1 to 469, and exhibits a molecular weight of 55.1 kilodaltons (kDa). For purification and detection purposes, a 20-amino acid Histidine tag is attached to the N-terminus of the ETS2 protein. The purification process involves proprietary chromatographic techniques to ensure high purity.
Physical Appearance
The solution is sterile-filtered, resulting in a clear and colorless liquid.
Formulation
The ETS2 protein solution has a concentration of 1 milligram per milliliter (mg/ml) and is prepared in a buffer containing 20 millimolar (mM) Tris-HCl at a pH of 8.0, 10% glycerol, and 0.4 molar (M) urea.
Stability
For short-term storage (2-4 weeks), the ETS2 protein solution should be kept refrigerated at 4 degrees Celsius. For extended storage, it is recommended to freeze the solution at -20 degrees Celsius. To further enhance stability during long-term storage, adding a carrier protein like bovine serum albumin (BSA) or human serum albumin (HSA) at a concentration of 0.1% is advisable. Repeated freezing and thawing of the solution should be avoided to preserve protein integrity.
Purity
Analysis by SDS-PAGE reveals that the purity of the ETS2 protein is greater than 85%.
Synonyms
Protein C-ets-2, ETS2, V-Ets Avian Erythroblastosis Virus E26 Oncogene 2, V-ets erythroblastosis virus E26 oncogene homolog 2, ETS2IT1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MNDFGIKNMD QVAPVANSYR GTLKRQPAFD TFDGSLFAVF PSLNEEQTLQ EVPTGLDSIS HDSANCELPL LTPCSKAVMS QALKATFSGF KKEQRRLGIP KNPWLWSEQQ VCQWLLWATN EFSLVNVNLQ RFGMNGQMLC NLGKERFLEL APDFVGDILW EHLEQMIKEN QEKTEDQYEE NSHLTSVPHW INSNTLGFGT EQAPYGMQTQ NYPKGGLLDS MCPASTPSVL SSEQEFQMFP KSRLSSVSVT YCSVSQDFPG SNLNLLTNNS GTPKDHDSPE NGADSFESSD SLLQSWNSQS SLLDVQRVPS FESFEDDCSQ SLCLNKPTMS FKDYIQERSD PVEQGKPVIP AAVLAGFTGS GPIQLWQFLL ELLSDKSCQS FISWTGDGWE FKLADPDEVA RRWGKRKNKP KMNYEKLSRG LRYYYDKNII HKTSGKRYVY RFVCDLQNLL GFTPEELHAI LGVQPDTED.

Product Science Overview

Introduction

The V-Ets Avian Erythroblastosis Virus E26 Oncogene 2, commonly referred to as ETS2, is a member of the ETS (E-twenty-six) family of transcription factors. These transcription factors are known for their role in regulating gene expression, particularly in processes related to cell development, differentiation, and proliferation. ETS2, like other ETS family members, contains a conserved ETS DNA-binding domain that allows it to bind to specific DNA sequences and regulate the transcription of target genes.

Historical Context

The ETS family of transcription factors was first identified in the context of avian erythroblastosis virus, where the E26 oncogene was found to be responsible for the transformation of erythroblasts into cancerous cells. The discovery of ETS2 and its homologs has since expanded our understanding of the role of these transcription factors in both normal cellular processes and oncogenesis.

Structure and Function

ETS2 is a protein-coding gene that encodes a transcription factor involved in various cellular processes. The protein contains several functional domains, including:

  • ETS DNA-binding domain: This domain is responsible for binding to specific DNA sequences and regulating the transcription of target genes.
  • Pointed domain: This domain is involved in protein-protein interactions and is essential for the formation of transcriptional complexes.

ETS2 regulates the expression of numerous genes involved in cell cycle control, apoptosis, and differentiation. It plays a critical role in stem cell development, cellular senescence, and tumorigenesis .

Role in Disease

ETS2 has been implicated in various diseases, including cancer. Overexpression or dysregulation of ETS2 can lead to aberrant cell proliferation and tumorigenesis. For example, increased expression of ETS2 has been associated with certain types of leukemia and solid tumors . Additionally, ETS2 has been linked to other conditions such as nut allergy and choriocarcinoma .

Research and Therapeutic Potential

Research on ETS2 continues to uncover its role in various biological processes and diseases. Understanding the mechanisms by which ETS2 regulates gene expression and contributes to disease progression is crucial for developing targeted therapies. Inhibitors of ETS2 and its downstream signaling pathways are being explored as potential therapeutic agents for cancer and other diseases.

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