ESM1 Human

Endothelial Cell-Specific Molecule 1 Human Recombinant
Cat. No.
BT1514
Source
Escherichia Coli.
Synonyms
Endothelial cell-specific molecule 1, ESM-1, ESM1, endocan.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ESM1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 188 amino acids (20-184 a.a.) and having a molecular mass of 20.5kDa.
ESM1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Endothelial cell-specific molecule 1 (ESM1), primarily found in human lung and kidney tissues, is a proteoglycan secreted by endothelial cells. Its mRNA expression is modulated by inflammatory cytokines. ESM1 plays a crucial role in the interactions between lung endothelial cells and leukocytes. Moreover, its expression is observed in various epithelia and adipocytes. Involved in angiogenesis, ESM1 promotes angiogenic sprouting. While TNF alpha, IL1 beta, and lipopolysaccharide upregulate its expression, IFN gamma downregulates it. Overexpression of ESM1 in genetically engineered cells leads to tumor formation, suggesting its potential involvement in the pathophysiology of tumor growth in vivo.
Description
Recombinant human ESM1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 188 amino acids (specifically, residues 20-184). With a molecular weight of 20.5 kDa, it features a 23 amino acid His-tag fused at the N-terminus. The purification process involves proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The ESM1 protein solution is provided at a concentration of 1 mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 2M Urea, and 10% glycerol.
Stability
For optimal storage, maintain the product at 4°C if the entire vial will be used within 2-4 weeks. For extended storage, it is recommended to store the product frozen at -20°C. To further enhance long-term stability, consider adding a carrier protein (either 0.1% HSA or BSA). Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the protein is determined to be greater than 90.0% using SDS-PAGE analysis.
Synonyms
Endothelial cell-specific molecule 1, ESM-1, ESM1, endocan.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSWSNNYAV DCPQHCDSSE CKSSPRCKRT VLDDCGCCRV CAAGRGETCY RTVSGMDGMK CGPGLRCQPS NGEDPFGEEF GICKDCPYGT FGMDCRETCN CQSGICDRGT GKCLKFPFFQ YSVTKSSNRF VSLTEHDMAS GDGNIVREEV VKENAAGSPV MRKWLNPR.

Product Science Overview

Molecular Characteristics and Expression

ESM-1 is a 50 kDa proteoglycan that is mainly expressed in the endothelial cells of human lung and kidney tissues . The expression of the ESM-1 gene is regulated by inflammatory cytokines, suggesting its role in endothelium-dependent pathological disorders . ESM-1 can be detected in the human bloodstream and is involved in various physiological and pathological processes, including inflammation, angiogenesis, and lymphangiogenesis .

Role in Disease and Therapeutic Potential

ESM-1 has been identified as a specific biomarker of tip cells during neoangiogenesis, which is the formation of new blood vessels from pre-existing ones . Its aberrant expression is associated with several pathological conditions, including cancer, sepsis, kidney diseases, and cardiovascular diseases . In cancer, ESM-1 promotes tumor progression and metastasis by regulating tumor cell proliferation, migration, invasion, and drug resistance . It is also involved in the tumor microenvironment, influencing inflammation and angiogenesis .

Clinical and Pre-Clinical Applications

Due to its significant role in various diseases, ESM-1 is being explored as a diagnostic and prognostic indicator. Its potential as a therapeutic target is also being investigated, particularly in cancer therapy . The recombinant form of ESM-1 (human recombinant) is used in research to study its functions and therapeutic potential.

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