ESM1 Human, SF9
Sf9, Baculovirus cells.
Endothelial Cell Specific Molecule 1, Endothelial Cell-Specific Molecule 1, ESM-1, Endocan.
Greater than 95.0% as determined by SDS-PAGE.
Description
ESM1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 174 amino acids (20-184a.a.) and having a molecular mass of 19.2kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). ESM1 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
ESM1, also known as Endothelial cell-specific molecule 1, is a protein encoded by the ESM1 gene in humans. This secreted protein is highly expressed in the endothelial cells of human kidney and lung tissues. Due to its regulation by cytokines, ESM1 is believed to be involved in endothelium-dependent pathological diseases.
Produced in Sf9 insect cells using a baculovirus expression system, ESM1 is a single, glycosylated polypeptide chain. This recombinant protein consists of 174 amino acids (20-184 a.a.), with a molecular weight of 19.2 kDa. However, it appears between 18-28 kDa on SDS-PAGE. The protein is expressed with a 9 amino acid His tag at the C-terminus and purified through proprietary chromatographic techniques.
The ESM1 protein solution is provided at a concentration of 0.25 mg/ml in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
The purity of the ESM1 protein is greater than 95.0% as determined by SDS-PAGE analysis.
Endothelial Cell Specific Molecule 1, Endothelial Cell-Specific Molecule 1, ESM-1, Endocan.
Sf9, Baculovirus cells.
ADLWSNNYAV DCPQHCDSSE CKSSPRCKRT VLDDCGCCRV CAAGRGETCY RTVSGMDGMK CGPGLRCQPS NGEDPFGEEF GICKDCPYGT FGMDCRETCN CQSGICDRGT GKCLKFPFFQ YSVTKSSNRF VSLTEHDMAS GDGNIVREEV VKENAAGSPV MRKWLNPRHH HHHH.
Product Science Overview
ESM-1 is secreted into the bloodstream and can be detected in various tissues, including the lungs and kidneys . It plays a significant role in angiogenesis, promoting the sprouting of new blood vessels . The expression of ESM-1 is regulated by cytokines, suggesting its involvement in endothelium-dependent pathological disorders .
ESM-1 is involved in several critical biological processes:
- Cancer Progression: ESM-1 promotes cancer progression and metastasis by regulating tumor cell proliferation, migration, invasion, and drug resistance .
- Inflammation and Angiogenesis: It is involved in the tumor microenvironment, influencing inflammation, angiogenesis, and lymphangiogenesis .
- Endothelial Cell-Leukocyte Interactions: ESM-1 has implications in lung endothelial cell-leukocyte interactions, which are crucial for immune responses .
Due to its aberrant expression under pathological conditions, ESM-1 serves as a diagnostic and prognostic indicator for various diseases, including cancer, sepsis, kidney diseases, and cardiovascular diseases . Its role in promoting angiogenesis and its presence in the bloodstream make it a potential target for therapeutic interventions, particularly in cancer treatment .
The recombinant form of ESM-1, produced using the Sf9 insect cell line, is used in research to study its functions and potential therapeutic applications. This recombinant protein retains the biological activity of the native molecule, making it a valuable tool for scientific investigations.
