ESAT6-CFP10
Escherichia Coli.
Early Secretory Target Mycobacterium Tuberculosis, ESAT-6
Sterile Filtered clear solution.
Protein is >95% pure as determined by 12% PAGE (coomassie staining).
Description
Recombinant Chimeric ESAT6-CFP10 produced in E. coli is fused to a 6 amino acid his tag at its C-terminus and purified by proprietary chromatographic technique.
Product Specs
Mycobacterium antigen ESAT6, found in low molecular weight fractions of the short-term-culture filtrate (ST-CF), is readily detectable in tuberculosis patients. ESAT-6, a potent T-cell antigen, and related proteins are exported through a specialized secretory system encoded by a cluster of genes. Notably, several of these genes also encode proteins strongly recognized by T cells, making the ESAT-6 systems 'immunogenicity islands'. Growing evidence suggests that these genes undergo selective pressure from the host's immune system. This antigen possesses numerous epitopes detectable in the serum of over 90% of tuberculosis patients. Vaccine development efforts based on ESAT-6 highlight the critical importance of adjuvant optimization, particularly when using dioctadecylammonium bromide and monophosphoryllipide. ESAT-6 holds significant potential as a diagnostic tool for differentiating between mycobacterial infection and BCG vaccination. Its primary applications include antibody production and the development of tuberculosis test systems. Early secretory antigen 6 (ESAT6) and cell filtrate protein 10 (CFP10) are two antigens secreted as a complex by the replicating Mycobacterium tuberculosis complex (MTC).
Recombinant Chimeric ESAT6-CFP10, produced in E. coli, is fused with a 6 amino acid histidine tag at its C-terminus and purified using a proprietary chromatographic method.
Sterile, clear solution.
The ESAT6-CFP10 solution is formulated in 25mM K2CO3, 0.02% NaN3, and PBS.
For short-term storage (up to 2-4 weeks), store the entire vial at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
The protein purity exceeds 95% as determined by 12% SDS-PAGE analysis with Coomassie blue staining.
This product is suitable for immunoassay applications.
Early Secretory Target Mycobacterium Tuberculosis, ESAT-6
Escherichia Coli.
Product Science Overview
ESAT6 is a potent T-cell antigen that has been isolated from low molecular weight fractions of the short-term culture filtrate (ST-CF) of Mycobacterium tuberculosis. It is recognized strongly by T cells and includes many epitopes detectable in the serum of most TB patients . The export of ESAT6 and related proteins requires a dedicated secretory apparatus encoded by a group of genes, several of which also code for proteins recognized by T cells . This antigen is highly immunogenic and is considered a potential diagnostic marker for differentiating between mycobacterial infection and BCG vaccination .
The chimeric recombinant ESAT6-CFP10 is produced in Escherichia coli and is fused to a 6 amino acid his tag at its C-terminus . This recombinant protein is purified using proprietary chromatographic techniques and is used in various immunoassays . The formulation of ESAT6-CFP10 includes 25mM K2CO3, 0.02% NaN3, and PBS . It is stored at 4°C for short-term use and at -20°C for long-term storage .
The ESAT6-CFP10 chimeric recombinant protein is used in laboratory research, particularly in immunoassays to detect TB infection . It has been reported to be accurate and safe in identifying TB infection, with fewer adverse reactions compared to the traditional tuberculin skin test (TST) . In a clinical study, the ESAT6-CFP10 skin test showed significantly fewer adverse reactions, such as bleeding, dermatodyschroia, and swelling, compared to the TST .
