ERP44 Human

Endoplasmic Reticulum Protein 44 Human Recombinant
Cat. No.
BT1887
Source
Escherichia Coli.
Synonyms
PDIA10, TXNDC4, ER protein 44.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ERP44 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 415 amino acids (30-406 a.a.) and having a molecular mass of 48 kDa. The ERP44 is fused to a 38 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
ERP44, residing in the endoplasmic reticulum, functions as a chaperone protein. Its role involves thiol-dependent retention within the secretory pathway, where it forms mixed disulfides with substrate proteins via its CRFS motif. This interaction contributes to the regulation of protein folding and transport. ERP44 also interacts with ITPR1, influencing its calcium channel activity. Furthermore, ERP44 plays a crucial role in managing oxidative protein folding within the endoplasmic reticulum.
Description
Recombinant Human ERP44, expressed in E. coli, is a non-glycosylated polypeptide chain lacking post-translational modifications. It consists of 415 amino acids, specifically residues 30 to 406, resulting in a molecular weight of 48 kDa. For purification and detection purposes, a 38 amino acid His-Tag is fused to the N-terminus. The protein has been purified using proprietary chromatographic methods.
Physical Appearance
The product is a clear and sterile solution without any color.
Formulation
The ERP44 protein is provided as a solution at a concentration of 1mg/ml. The solution is buffered with 20mM Tris-HCl at a pH of 7.5 and contains 10% glycerol as a stabilizing agent.
Stability
For short-term storage (2-4 weeks), the product should be kept at a refrigerated temperature of 4°C. For extended storage, it is recommended to store the protein in frozen aliquots at -20°C. To further enhance long-term stability, the addition of a carrier protein such as 0.1% HSA or BSA is advisable. Repeated freezing and thawing cycles should be avoided.
Purity
The purity of ERP44 is determined using SDS-PAGE analysis and is confirmed to be greater than 90%.
Synonyms
PDIA10, TXNDC4, ER protein 44.
Source
Escherichia Coli.
Amino Acid Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMEI TSLDTENIDE ILNNADVALV NFYADWCRFS QMLHPIFEEA SDVIKEEFPN
ENQVVFARVD CDQHSDIAQR YRISKYPTLK LFRNGMMMKR EYRGQRSVKA LADYIRQQKS DPIQEIRDLA EITTLDRSKR NIIGYFEQKD
SDNYRVFERV ANILHDDCAF LSAFGDVSKP ERYSGDNIIY KPPGHSAPDM VYLGAMTNFD VTYNWIQDKC VPLVREITFE NGEELTEEGL
PFLILFHMKE DTESLEIFQN EVARQLISEK GTINFLHADC DKFRHPLLHI QKTPADCPVI AIDSFRHMYV FGDFKDVLIP GKLKQFVFDL
HSGKLHREFH HGPDPTDTAP GEQAQDVASS PPESSFQKLA PSEYRYTLLR DRDEL.

Product Science Overview

Structure and Function

ERP44 is characterized by:

  • An inferred N-terminal signal peptide
  • A catalytically active thioredoxin (TRX) domain
  • Two TRX-like domains
  • A C-terminal ER-retention sequence

These structural features enable ERP44 to function as a pH-regulated chaperone, mediating thiol-dependent retention in the early secretory pathway . It forms mixed disulfides with substrate proteins through its conserved CRFS motif . Additionally, ERP44 inhibits the calcium channel activity of ITPR1 and is involved in the control of oxidative protein folding in the ER .

Biological Significance

ERP44 is essential for retaining ERO1A and ERO1B in the ER, which are crucial for oxidative protein folding . It also plays a role in various cellular processes, including:

  • Calcium ion binding
  • Protein disulfide isomerase activity
Clinical Relevance

Mutations or dysregulation of ERP44 have been associated with diseases such as Multiple Sulfatase Deficiency and Amyotrophic Lateral Sclerosis 15 with or without Frontotemporal Dementia . Understanding the function and regulation of ERP44 can provide insights into these conditions and potential therapeutic targets.

Recombinant ERP44

Recombinant human ERP44 is typically expressed in HEK293 cells and is used for research purposes . It is supplied as a highly purified protein, often with a His tag for easy purification and detection . The recombinant protein retains the functional properties of the native protein, making it a valuable tool for studying ERP44’s role in cellular processes and disease mechanisms.

For more detailed information, you can refer to sources like GeneCards and UniProt.

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