ERP27 Human

Endoplasmic Reticulum Protein 27 Human Recombinant
Cat. No.
BT1801
Source
Escherichia Coli.
Synonyms
Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, ERP27, PDIA8, C12orf46, FLJ32115.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

ERP27 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 269 amino acids (26-273 a.a.) and having a molecular mass of 30kDa. The ERP27 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Endoplasmic reticulum protein 27kDa (ERP27) is a widely expressed protein that resides in the endoplasmic reticulum (ER). It is believed to play a role in protein processing within the ER, potentially acting as a protease, protein disulfide isomerase, thiol-disulfide oxidase, or phospholipase. However, ERP27 lacks the typical CXXC active site motif found in many redox-active enzymes, suggesting it might not be directly involved in catalyzing redox reactions.
Description
This product consists of the human ERP27 protein produced in E. coli. A 21 amino acid His tag is attached to the N-terminus to facilitate purification. The protein is a single, non-glycosylated polypeptide chain encompassing amino acids 26 to 273 of the ERP27 sequence. With a molecular weight of 30kDa, the recombinant ERP27 is purified using proprietary chromatographic methods.
Physical Appearance
A clear and colorless solution, sterilized by filtration.
Formulation
The ERP27 is supplied in a solution at a concentration of 1mg/ml. The solution also contains 20mM Tris buffer (pH 8.0), 10% glycerol, and 1mM DTT.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For longer storage, it is recommended to freeze the product at -20°C. To ensure stability during long-term storage, adding a carrier protein like 0.1% HSA or BSA is advisable. Avoid repeated freezing and thawing of the product.
Purity
The purity of the ERP27 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, ERP27, PDIA8, C12orf46, FLJ32115.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MEVEKSSDGP GAAQEPTWLT DVPAAMEFIA ATEVAVIGFF QDLEIPAVPI LHSMVQKFPG VSFGISTDSE VLTHYNITGN TICLFRLVDN EQLNLEDEDI ESIDATKLSR FIEINSLHMV TEYNPVTVIG LFNSVIQIHL LLIMNKASPE YEENMHRYQK AAKLFQGKIL FILVDSGMKE NGKVISFFKL KESQLPALAI YQTLDDEWDT LPTAEVSVEH VQNFCDGFLS GKLLKENRES EGKTPKVEL.

Product Science Overview

Structure and Localization

ERP27 has a distinct structure characterized by:

  • An N-terminal signal sequence that directs the protein to the endoplasmic reticulum (ER).
  • Two thioredoxin (TRX)-like domains, which are typically involved in redox reactions but in this case, do not exhibit catalytic activity.
  • A C-terminal ER-retention sequence that ensures the protein remains within the ER .
Function

ERP27 plays a role in the unfolded protein response (UPR), a cellular stress response related to the ER. It specifically binds to unfolded proteins and may recruit other protein disulfide isomerases, such as PDIA3, to assist in protein folding. This binding is facilitated by a hydrophobic pocket in the C-terminal domain of ERP27 .

Expression and Pathways

ERP27 is expressed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle. It is involved in pathways such as the photodynamic therapy-induced unfolded protein response .

Clinical Significance

Mutations or dysregulation of the ERP27 gene have been associated with certain diseases, including Deafness, Autosomal Dominant 59. This highlights the importance of ERP27 in maintaining cellular homeostasis and proper protein folding within the ER .

Research and Applications

Human recombinant ERP27 is used in research to study its role in the ER and its interactions with other proteins. Understanding ERP27’s function and mechanisms can provide insights into the development of therapeutic strategies for diseases related to protein misfolding and ER stress.

For more detailed information, you can refer to resources like GeneCards and The Human Protein Atlas.

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Source
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THE BIOTEK
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