EPOR Human

Erythropoietin Receptor Human Recombinant
Cat. No.
BT5498
Source
Sf9, Baculovirus cells.
Synonyms

Erythropoietin Receptor, EPO-R, EPOR.

Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EPOR Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 232 amino acids (25-250 a.a) and having a molecular mass of 25.6kDa. (Migrates at 28-40kDa on SDS-PAGE under reducing conditions). EPOR is fused to an 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The erythropoietin receptor, also known as EPOR, plays a crucial role in the production of red blood cells. It facilitates the proliferation and differentiation of erythroblasts upon binding with erythropoietin (EPO). This binding triggers the activation of Jak2 tyrosine kinase, which in turn sets off several intracellular signaling pathways such as Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors. These pathways are essential for erythroid cell survival and development. Mutations in the EPOR gene can lead to blood disorders like erythroleukemia and familial erythrocytosis. Moreover, dysregulation of EPOR can impact the growth of certain tumors.
Description
Recombinant human EPOR, produced in Sf9 insect cells using a baculovirus expression system, is a single glycosylated polypeptide chain. This protein consists of 232 amino acids (25-250 a.a), has a molecular weight of 25.6 kDa, and appears as a band between 28-40 kDa on SDS-PAGE under reducing conditions. This discrepancy in observed versus calculated molecular weight is attributed to glycosylation. The EPOR protein is engineered with a 6-amino acid His-tag at the C-terminus to facilitate purification using chromatographic techniques.
Physical Appearance
The purified EPOR protein solution is clear and sterile-filtered.
Formulation
The EPOR protein is supplied in a solution with a concentration of 0.5 mg/ml. The storage buffer consists of Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the EPOR protein solution can be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) can enhance protein stability during long-term storage. To maintain the integrity of the protein, avoid repeated freeze-thaw cycles.
Purity
The purity of the EPOR protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms

Erythropoietin Receptor, EPO-R, EPOR.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence
APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT PSDLDPHHHH HH.

Product Science Overview

Introduction

The Erythropoietin Receptor (EpoR) is a crucial component in the regulation of red blood cell production. It is a protein found on the surface of erythroid progenitor cells in the bone marrow. The receptor is activated by erythropoietin (EPO), a hormone primarily produced by the kidneys in response to hypoxia (low oxygen levels). The interaction between EPO and EpoR is essential for the survival, proliferation, and differentiation of erythroid progenitor cells into mature red blood cells.

Structure and Function

EpoR is a member of the cytokine receptor family and is composed of a single polypeptide chain. The receptor has an extracellular domain that binds to EPO, a transmembrane domain, and an intracellular domain that transduces the signal into the cell. Upon binding of EPO to EpoR, the receptor undergoes a conformational change that activates intracellular signaling pathways, including the JAK2/STAT5, PI3K/Akt, and MAPK pathways. These pathways lead to the transcription of genes involved in erythroid cell survival and proliferation .

Recombinant Erythropoietin Receptor

Recombinant human erythropoietin receptor (rhEpoR) is produced using recombinant DNA technology. This involves inserting the gene encoding EpoR into a suitable host cell, such as Chinese hamster ovary (CHO) cells, which then express the receptor. The recombinant receptor is used in various research and clinical applications to study the mechanisms of erythropoiesis and to develop therapeutic agents for anemia and other related conditions .

Clinical Applications

The recombinant EpoR is used in the development of erythropoiesis-stimulating agents (ESAs), which are used to treat anemia, particularly in patients with chronic kidney disease, cancer, and those undergoing chemotherapy. ESAs mimic the action of natural EPO by binding to EpoR and stimulating red blood cell production. This reduces the need for blood transfusions and improves the quality of life for patients with anemia .

Research and Development

Research on EpoR has led to significant advancements in understanding the molecular mechanisms of erythropoiesis. Studies have shown that EpoR is not only expressed in erythroid progenitor cells but also in other tissues, including the brain, heart, and kidneys. This suggests that EpoR may have additional roles beyond erythropoiesis, such as neuroprotection and tissue repair .

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