EREG Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Epiregulin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 49 amino acids and having a molecular mass of 5.6 kDa.
Epiregulin is purified by proprietary chromatographic techniques.
Product Specs
Recombinant Human Epiregulin, produced in E. coli, is a single, non-glycosylated polypeptide chain with 49 amino acids and a molecular mass of 5.6 kDa. The purification process involves proprietary chromatographic techniques.
The lyophilization of Epiregulin was carried out from a 0.5mg/ml solution containing 20mM PBS buffer at pH 7.4 and 20mM sodium chloride.
(a) RP-HPLC analysis.
(b) SDS-PAGE analysis.
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Product Science Overview
Recombinant human epiregulin is typically produced in E. coli and consists of amino acids Val63 to Leu108 . The recombinant protein is often purified to a high degree, with a purity greater than 97% as determined by SDS-PAGE under reducing conditions . The molecular weight of epiregulin is approximately 5.4 kDa .
Epiregulin plays a crucial role in cell proliferation and differentiation. It induces the proliferation of keratinocytes and corneal epithelial cells, promotes oocyte maturation, and may function in blastocyst implantation during pregnancy . Epiregulin is unique among EGF family members as it binds to both ErbB1/HER1 and ErbB4/HER4 receptors . This binding induces receptor homodimerization or heterodimerization with other Erb/Her receptors, activating downstream signaling pathways such as ERK1/2 and PI3K/Akt .
Recombinant human epiregulin is used in various research applications, including cell proliferation assays. For instance, it has been shown to stimulate the proliferation of Balb/3T3 mouse embryonic fibroblast cells with an effective dose (ED50) ranging from 0.125 to 0.75 ng/mL . Similarly, it promotes the proliferation of MCF 10A cells with an ED50 between 0.10-0.85 ng/mL .
Recombinant human epiregulin is typically lyophilized and can be reconstituted in sterile PBS. It is stable for up to 12 months when stored at -20 to -70°C in its lyophilized form . After reconstitution, it remains stable for 1 month at 2 to 8°C and for 3 months at -20 to -70°C under sterile conditions .
