EPHX1 Human, Sf9
Sf9, Insect cells.
Epoxide hydrolase 1, Epoxide hydratase, Microsomal epoxide hydrolase, Meh, EPHX1, EPHX, EPOX, Epoxide Hydrolase 1 Microsomal, Microsomal Epoxide Hydrolase,
EC 3.3.2.9, HYL1
Greater than 85% as determined by SDS-PAGE.
Description
EPHX1 produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 442 amino acids (21-455 a.a.) and having a molecular mass of 51.5kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).
EPHX1 is expressed with an 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Epoxide hydrolase 1, Epoxide hydratase, Microsomal epoxide hydrolase, Meh, EPHX1, EPHX, EPOX, Epoxide Hydrolase 1 Microsomal, Microsomal Epoxide Hydrolase,
EC 3.3.2.9, HYL1
Sf9, Insect cells.
MRDKEETLPL EDGWWGPGTR SAAREDDSIR PFKVETSDEE IHDLHQRIDK FRFTPPLEDS CFHYGFNSNY LKKVISYWRN EFDWKKQVEI LNRYPHFKTK IEGLDIHFIH VKPPQLPAGH TPKPLLMVHG WPGSFYEFYK IIPLLTDPKN HGLSDEHVFE VICPSIPGYG FSEASSKKGF NSVATARIFY KLMLRLGFQE FYIQGGDWGS LICTNMAQLV PSHVKGLHLN MALVLSNFST LTLLLGQRFG RFLGLTERDV ELLYPVKEKV FYSLMRESGY MHIQCTKPDT VGSALNDSPV GLAAYILEKF STWTNTEFRY LEDGGLERKF SLDDLLTNVM LYWTTGTIIS SQRFYKENLG QGWMTQKHER MKVYVPTGFS AFPFELLHTP EKWVRFKYPK LISYSYMVRG GHFAAFEEPE LLAQDIRKFL SVLERQHHHH HH.
Product Science Overview
Epoxide Hydrolase 1 (EH1), also known as microsomal epoxide hydrolase (mEH), is an enzyme encoded by the EPHX1 gene located on chromosome 1 (1q42.12) in humans . This enzyme plays a crucial role in the metabolism of both xenobiotic and endogenous compounds by converting epoxides to diols, which are more hydrophilic and can be easily excreted from the body .
The human microsomal epoxide hydrolase is a membrane-bound enzyme consisting of 455 amino acids . It is primarily found in the endoplasmic reticulum of cells and is involved in the detoxification of reactive epoxides formed during the metabolism of various compounds, including drugs, environmental pollutants, and endogenous lipids .
EH1 is a vital biotransformation enzyme that catalyzes the hydrolysis of epoxides to trans-dihydrodiols . This reaction is essential for the detoxification of potentially harmful epoxides, which can form adducts with DNA and proteins, leading to cellular damage and mutagenesis. By converting these reactive epoxides to less harmful diols, EH1 helps protect cells from oxidative stress and chemical-induced toxicity .
The activity of EH1 has been linked to various diseases and conditions. For instance, genetic polymorphisms in the EPHX1 gene can result in altered enzyme activity, which has been associated with an increased risk of developing certain cancers, preeclampsia, and neurological disorders . Additionally, EH1 has been studied as a potential biomarker for diseases such as metastatic cancer and viral hepatitis .
The recombinant form of human microsomal epoxide hydrolase (EH1) is often produced using the baculovirus expression system in sf9 insect cells. This system allows for the high-level expression of functional EH1, which can be used for various research and therapeutic applications . The recombinant enzyme retains its catalytic activity and can be used to study the enzyme’s structure, function, and role in disease processes.
Recombinant EH1 is used in various applications, including:
- Drug Metabolism Studies: Understanding how drugs are metabolized and identifying potential drug-drug interactions.
- Toxicology Research: Investigating the detoxification pathways of environmental pollutants and other xenobiotics.
- Biomarker Development: Developing assays to measure EH1 activity as a potential biomarker for disease diagnosis and prognosis .
