EPHX1 Antibody

Epoxide hydrolase 1 (EPHX1) is an enzyme that plays a crucial role in the metabolism of both endogenous and exogenous compounds. It is encoded by the EPHX1 gene in humans and is involved in the detoxification of potentially harmful epoxides by converting them into less reactive and more water-soluble dihydrodiols .

Microsomal epoxide hydrolase 1 (EPHX1) was first isolated by Watabe and Kanehira from rabbit liver and later purified from human liver . EPHX1 belongs to the family of α/β hydrolases and is known for its ability to convert epoxides to diols .

EPHX1 plays a significant role in both the activation and detoxification of exogenous chemicals such as polycyclic aromatic hydrocarbons . It catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive dihydrodiols by the trans addition of water . This enzyme is also involved in the metabolism of endogenous lipids, such as epoxide-containing fatty acids .

EPHX1 protein is predominantly found in the membrane fraction of the endoplasmic reticulum of eukaryotic cells . In mammals, its expression is highest in the liver, followed by the adrenal gland, lung, kidney, and intestine . It is also present in bronchial epithelial cells and the upper gastrointestinal tract .

The expression of EPHX1 is individually variable among humans and can be modestly induced by chemicals such as phenobarbital, β-naphtoflavone, benzanthracene, and trans-stilbene oxide . Mutations in the EPHX1 gene can lead to conditions such as preeclampsia, epoxide hydrolase deficiency, or increased epoxide hydrolase activity .

Mouse anti-human EPHX1 antibodies are used in research to study the expression and function of EPHX1 in various tissues. These antibodies are crucial for understanding the role of EPHX1 in different biological processes and its involvement in various diseases.