EFNA1 Human, Sf9

Ephrin A1 Human Recombinant, Sf9
Cat. No.
BT2677
Source
Sf9, Baculovirus cells.
Synonyms

Ephrin A1, Immediate Early Response Protein B61, TNF Alpha-Induced Protein 4, Ephrin-A1, TNFAIP4, LERK-1, EPLG1, LERK1, Tumor Necrosis Factor, Alpha-Induced Protein 4, Eph-Related Receptor Tyrosine Kinase Ligand 1, EPH-Related Receptor Tyrosine Kinase Ligand 1, Tumor Necrosis Factor Alpha-Induced Protein 4, Ligand Of Eph-Related Kinase 1, ECKLG, EFL1, B61, EPH-related receptor tyrosine kinase ligand 1, LERK-1.

Appearance
Sterile Filtered colorless solution.
Purity

Greater than 90% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

EFNA1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 406 amino acids (19-182a.a.) and having a molecular mass of 46.6kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
EFNA1 is expressed with a 242 amino acid hIgG-His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
EFNA1 is part of the ephrin (EPH) family, which constitutes the largest group of receptor protein kinases. These kinases play a crucial role in the development and functionality of the nervous system.
Description
Produced in Sf9 Baculovirus cells, EFNA1 is a single, glycosylated polypeptide chain. It comprises 406 amino acids (specifically, amino acids 19-182a.a.) and has a molecular mass of 46.6kDa. On SDS-PAGE, the molecular size will appear within the range of 40-57kDa. This EFNA1 protein is expressed with a 242 amino acid hIgG-His-tag located at the C-Terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A colorless solution that has undergone sterile filtration.
Formulation
The EFNA1 protein solution has a concentration of 0.5mg/ml. It is prepared with 10% glycerol and Phosphate Buffered Saline, maintaining a pH of 7.4.
Stability
For optimal storage, keep the solution at 4°C if the entire vial will be used within 2-4 weeks. For longer storage periods, freeze the solution at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. To preserve protein integrity, avoid repeated freeze-thaw cycles.
Purity
The purity of EFNA1 is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms

Ephrin A1, Immediate Early Response Protein B61, TNF Alpha-Induced Protein 4, Ephrin-A1, TNFAIP4, LERK-1, EPLG1, LERK1, Tumor Necrosis Factor, Alpha-Induced Protein 4, Eph-Related Receptor Tyrosine Kinase Ligand 1, EPH-Related Receptor Tyrosine Kinase Ligand 1, Tumor Necrosis Factor Alpha-Induced Protein 4, Ligand Of Eph-Related Kinase 1, ECKLG, EFL1, B61, EPH-related receptor tyrosine kinase ligand 1, LERK-1.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

ADPDRHTVFW NSSNPKFRNE DYTIHVQLND YVDIICPHYE DHSVADAAME QYILYLVEHE EYQLCQPQSK DQVRWQCNRP SAKHGPEKLS EKFQRFTPFT LGKEFKEGHS YYYISKPIHQ HEDRCLRLKV TVSGKITHSP QAHVNPQEKR LAADDPEVRV LHSIGHSLEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK HHHHHH

 

Product Science Overview

Structure and Production

Ephrin A1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 406 amino acids (19-182a.a.) and has a molecular mass of approximately 46.6 kDa . The molecular size on SDS-PAGE appears at approximately 40-57 kDa . This recombinant protein is expressed with a 242 amino acid hIgG-His-tag at the C-terminus and is purified using proprietary chromatographic techniques .

Physical Properties
  • Formulation: Ephrin A1 protein solution (0.5 mg/ml) contains 10% glycerol and Phosphate Buffered Saline (pH 7.4) .
  • Appearance: Sterile filtered colorless solution .
  • Stability: The protein should be stored at 4°C if used within 2-4 weeks. For longer storage, it should be frozen at -20°C, and it is recommended to add a carrier protein (0.1% HSA or BSA) to avoid multiple freeze-thaw cycles .
Biological Function

Ephrin A1 is involved in several key biological processes, including:

  • Nervous System Development: Ephrin A1 and its receptors are essential for the proper development and function of the nervous system .
  • Cell Signaling: Ephrin A1 interacts with Eph receptors to mediate cell signaling, which is crucial for cell positioning and tissue architecture .
Applications

Recombinant Ephrin A1 is widely used in research to study its role in cell signaling, development, and disease. It is particularly valuable in understanding the mechanisms of nervous system development and the potential therapeutic applications in neurodegenerative diseases and cancer .

Handling and Usage
  • Purity: Greater than 90% as determined by SDS-PAGE .
  • Safety: ProSpec’s products, including Ephrin A1, are intended for laboratory research use only and should not be used as drugs, agricultural or pesticidal products, food additives, or household chemicals .

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