EFNA1 Human, HEK
HEK293 cells.
Ephrin-A1, EPLG1, TNFAIP4, LERK1, EFL1, ECKLG, EPH-related receptor tyrosine kinase ligand 1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, TNF alpha-induced protein 4, ligand of eph-related kinase 1, tumor necrosis factor, alpha-induced protein 4.
Greater than 95.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
EFNA1 Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain (a.a 19-182) containing 170 amino acids including a 6 a.a C-terminal His tag. The total molecular mass is 20.2kDa (calculated).
Product Specs
EFNA1, a member of the ephrin (EPH) family, plays a crucial role in the central nervous system, contributing to its development and functionality. The EPH subfamily, known as the largest group of receptor protein kinases, is integral to these processes.
Recombinant EFNA1 Human, produced in HEK293 cells, is a single, glycosylated polypeptide chain with a molecular weight of 20.2kDa (calculated). It comprises 170 amino acids, including a 6 a.a C-terminal His tag, spanning from amino acid positions 19 to 182.
EFNA1 is supplied as a lyophilized powder, filtered through a 0.4 μm filter. The protein was initially prepared in a phosphate buffered saline solution at a pH of 7.5 with a 5% (w/v) trehalose concentration before lyophilization. The initial concentration before lyophilization was 0.5mg/ml.
To create a working stock solution, add deionized water to the lyophilized pellet until it reaches a concentration of approximately 0.5mg/ml. Ensure the pellet is fully dissolved before use. This product is not sterile; therefore, filter it through a sterile filter before introducing it to cell cultures.
Store the lyophilized protein at -20°C. After reconstitution, aliquot the protein solution to minimize freeze-thaw cycles. The reconstituted protein remains stable at 4°C for a limited period and exhibits consistent quality for at least one week when stored at this temperature.
The purity of this product is greater than 95.0%, as determined by SDS-PAGE analysis.
Ephrin-A1, EPLG1, TNFAIP4, LERK1, EFL1, ECKLG, EPH-related receptor tyrosine kinase ligand 1, Immediate early response protein B61, Tumor necrosis factor alpha-induced protein 4, TNF alpha-induced protein 4, ligand of eph-related kinase 1, tumor necrosis factor, alpha-induced protein 4.
HEK293 cells.
DRHTVFWNSS NPKFRNEDYT IHVQLNDYVD IICPHYEDHS VADAAMEQYI LYLVEHEEYQ LCQPQSKDQV RWQCNRPSAK HGPEKLSEKF QRFTPFTLGK EFKEGHSYYY ISKPIHQHED RCLRLKVTVS GKITHSPQAH DNPQEKRLAA DDPEVRVLHS IGHS HHHHHH.
Product Science Overview
Ephrin A1 is ubiquitously expressed in various tissues and cells, including those of the immune system. It interacts with Eph receptors to mediate changes in cellular shape, motility, migration, and proliferation. These interactions are essential for normal cellular processes during development and adult tissue homeostasis .
Recombinant human Ephrin A1 is produced using HEK293 cells, a human embryonic kidney cell line. The DNA sequence encoding human Ephrin A1 (NP_004419.2) is expressed with a polyhistidine tag at the C-terminus. The recombinant protein is typically lyophilized from sterile PBS, pH 7.4, and may include protectants such as trehalose, mannitol, and Tween80 before lyophilization .
Recombinant human Ephrin A1 is stable for up to twelve months when stored at -20°C to -80°C under sterile conditions. It is recommended to aliquot the protein to avoid repeated freeze-thaw cycles .
Ephrin A1’s diverse roles in cellular processes and its potential as a therapeutic target make it a significant focus of biomedical research.
