EPHB1 Human
HEK293 Cells.
EPHB-1, Ephrin type-B receptor 1, Eph Receptor B1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, HEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, NETHek6, Tyrosine-protein kinase receptor EPH-2, EPHT2, soluble EPHB1 variant 1
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
EPHB1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 529 amino acids (18-540 a.a) and having a molecular mass of 59.2 kDa.
EPHB1 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
EPH Receptor B1 (EPHB1), a member of the ephrin receptor subfamily within the protein tyrosine kinase family, is one of 16 known receptors. EPHB1 demonstrates binding affinity for ephrin-B2, ephrin-B1, ephrin-A3, ephrin-A1, ephrin-B3, and ephrin-A4. It also interacts with tyrosine kinase and phosphorylates syndecan-2, a crucial step for syndecan-2 clustering and spine formation. Ephrin receptors and their corresponding ligands, ephrins, play essential roles in various developmental processes, particularly within the nervous system.
Recombinantly produced in HEK293 cells, EPHB1 Human Recombinant is a single, glycosylated polypeptide chain. This protein consists of 529 amino acids (amino acids 18-540), resulting in a molecular weight of 59.2 kDa. It features a 6-amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.
Sterile filtered, colorless solution.
The EPHB1 solution is provided at a concentration of 0.25 mg/mL in a buffer consisting of phosphate-buffered saline (pH 7.4) and 10% glycerol.
For short-term storage (2-4 weeks), the entire vial can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain protein integrity, avoid repeated freeze-thaw cycles.
Purity is determined to be greater than 95.0% using SDS-PAGE analysis.
Biological activity is evaluated through its binding ability in a functional ELISA utilizing Human EFNB1 (catalog number: pro-2543).
EPHB-1, Ephrin type-B receptor 1, Eph Receptor B1, ELK, EPH tyrosine kinase 2, EPH-like kinase 6, EK6, hEK6, HEK6, Neuronally-expressed EPH-related tyrosine kinase, NET, NETHek6, Tyrosine-protein kinase receptor EPH-2, EPHT2, soluble EPHB1 variant 1
HEK293 Cells.
MEETLMDTRT ATAELGWTAN PASGWEEVSG YDENLNTIRT YQVCNVFEPN QNNWLLTTFI NRRGAHRIYT EMRFTVRDCS SLPNVPGSCK ETFNLYYYET DSVIATKKSA FWSEAPYLKV DTIAADESFS QVDFGGRLMK VNTEVRSFGP LTRNGFYLAF QDYGACMSLL SVRVFFKKCP SIVQNFAVFP ETMTGAESTS LVIARGTCIP NAEEVDVPIK LYCNGDGEWM VPIGRCTCKP GYEPENSVAC KACPAGTFKA SQEAEGCSHC PSNSRSPAEA SPICTCRTGY YRADFDPPEV ACTSVPSGPR NVISIVNETS IILEWHPPRE TGGRDDVTYN IICKKCRADR RSCSRCDDNV EFVPRQLGLT ECRVSISSLW AHTPYTFDIQ AINGVSSKSP FPPQHVSVNI TTNQAAPSTV PIMHQVSATM RSITLSWPQP EQPNGIILDY EIRYYEKEHN EFNSSMARSQ TNTARIDGLR PGMVYVVQVR ARTVAGYGKF SGKMCFQTLT DDDYKSELRE QLPHHHHHH
Product Science Overview
EPHB1 is a transmembrane protein that consists of several domains:
- Extracellular domain: Responsible for binding to ephrin ligands.
- Transmembrane segment: Anchors the receptor in the cell membrane.
- Cytoplasmic domain: Contains the kinase activity necessary for signal transduction .
The receptor binds to ephrin-B1, ephrin-B2, and other ephrin ligands, initiating bidirectional signaling that affects both the receptor-expressing and ligand-expressing cells .
