EPHA3 Mouse
HEK293 Cells.
Cek4, EK4, End3, ETK1, Hek, Hek4, Mek4, Tyro4, ephrin type-A receptor 3, ephrin type-A receptor 3 isoform1, receptor protein-tyrosine kinase, EPH-like kinase 4, tyrosine-protein kinase TYRO4, tyrosine-protein kinase receptor ETK1, Eph receptor A3, AW492086.
Greater than 95% as determined by SDS-PAGE.
Description
EPHA3 Mouse Recombinant produced in HEK293 cells is a single, polypeptide chain containing 527 amino acids (21-541 a.a.) and having a molecular mass of 59.5kDa. EPHA3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Cek4, EK4, End3, ETK1, Hek, Hek4, Mek4, Tyro4, ephrin type-A receptor 3, ephrin type-A receptor 3 isoform1, receptor protein-tyrosine kinase, EPH-like kinase 4, tyrosine-protein kinase TYRO4, tyrosine-protein kinase receptor ETK1, Eph receptor A3, AW492086.
HEK293 Cells.
ELSPQPSNEV NLLDSKTIQG ELGWISYPSH GWEEISGVDE HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK FTLRDCNSIP LVLGTCKETF NLYYMESDDD HGVKFREHQF TKIDTIAADE SFTQMDLGDR ILKLNTEIRE VGPVNKKGFY LAFQDVGACV ALVSVRVYFK KCPFTVKNLA MFPDTVPMDS QSLVEVRGSC VNNSKEEDPP RMYCSTEGEW LVPIGKCTCN AGYEERGFIC QACRPGFYKA SDGAAKCAKC PPHSSTQEDG SMNCRCENNY FRAEKDPPSM ACTRPPSAPR NVISNINETS VILDWSWPLD TGGRKDITFN IICKKCGWNV RQCEPCSPNV RFLPRQLGLT NTTVTVTDLL AHTNYTFEID AVNGVSELSS PPRQYAAVSI TTNQAAPSPV MTIKKDRTSR NSISLSWQEP EHPNGIILDY EVKYYEKQEQ ETSYTILRAR GTNVTISSLK PDTTYVFQIR ARTAAGYGTN SRKFEFETSP DSFSISGENS HHHHHHH.
Product Science Overview
EPH Receptor A3, also known as EphA3, is a member of the Eph receptor family, which is the largest known family of receptor tyrosine kinases in mammals. EphA3 is also referred to by several other names, including Cek4, Mek4, Hek, Tyro4, and Hek4 . These receptors play a critical role in various developmental processes, particularly in the nervous system, and are key mediators of adult tissue homeostasis .
EphA3 is a glycosylated protein with a molecular weight of approximately 135 kDa . It has an extracellular region that includes a cysteine-rich domain and two fibronectin type III repeats, as well as a single kinase domain . EphA3 preferentially binds to ephrin-A5, a member of the ephrin ligand family . The interaction between Eph receptors and ephrin ligands is crucial for cell-cell communication, which influences cell positioning, shape, and movement during development .
EphA3 is expressed in various tissues during development, including the forebrain, retinal axons, some motor neurons in the spinal cord, and the heart . It is also found on some blood and solid tumor cells, as well as on astrocytes near injured nerve tissue . EphA3 regulates axonal guidance and organ formation, making it essential for proper nervous system development and function .
Recombinant mouse EphA3 is produced using various expression systems, such as HEK293 cells . The recombinant protein is often fused with a His-tag at the C-terminus to facilitate purification . The purified protein is used in various applications, including functional ELISA assays to measure its binding ability to human EFNA5 .
Recombinant mouse EphA3 is widely used in research to study its role in developmental processes and disease. It is particularly valuable for investigating the mechanisms of axonal guidance, organ formation, and tumor progression . Additionally, EphA3 and its ligands are being explored as potential therapeutic targets for various diseases, including cancer .
