EPGN Human
Escherichia Coli.
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Epigen Recombinant Human produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa.
Epigen is purified by proprietary chromatographic techniques.
Product Specs
Epidermal growth factor (EGF)-related polypeptide growth factor (EPGN) is a growth factor similar to EGF that interacts with the ErbB receptor-1 to initiate signaling. EPGN is found in various tissues, including the testes, liver, heart, and specific tumor cells. It promotes the growth of fibroblasts and epithelial cells. Human EPGN is initially produced as a glycosylated transmembrane precursor protein with a molecular weight of 14.7 kDa. This precursor protein undergoes proteolytic cleavage to yield the mature soluble form of EPGN.
Recombinant Human Epigen, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 72 amino acids. It has a molecular weight of 7.9 kDa. The purification process for Epigen involves the use of proprietary chromatographic methods.
The product appears as a white powder that has been sterilized by filtration and lyophilized (freeze-dried).
The lyophilization buffer for EPGN is 20mM PBS with a pH of 7.4 and 130mM sodium chloride.
To reconstitute the lyophilized Epigen, it is recommended to dissolve it in sterile 18MΩ-cm H₂O at a minimum concentration of 100µg/ml. This solution can then be further diluted with other aqueous solutions as needed.
Lyophilized Epigen remains stable at room temperature for a period of 3 weeks. However, for long-term storage, it is recommended to store it in a desiccated condition below -18°C. After reconstitution, EPGN should be stored at 4°C for a period of 2-7 days. For extended storage, it is advisable to store it below -18°C. To ensure optimal stability during long-term storage, it is recommended to add a carrier protein such as 0.1% HSA or BSA. Avoid repeated cycles of freezing and thawing.
The purity of the product is determined to be greater than 98.0% based on the following analyses:
(a) Reverse-phase high-performance liquid chromatography (RP-HPLC)
(b) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
The biological activity is assessed by measuring the dose-dependent stimulation of proliferation in murine Balb/3T3 cells. The anticipated ED₅₀ for this effect is in the range of 150-300 ng/ml. This corresponds to a specific activity of 3,334-6,667 international units per milligram (IU/mg).
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
Escherichia Coli.
AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA
Product Science Overview
Human Epigen cDNA encodes a type I transmembrane precursor glycoprotein consisting of 154 amino acid residues. The structure includes:
- A 22 amino acid signal peptide
- An 88 amino acid extracellular domain
- A 21 amino acid transmembrane domain
- A 23 amino acid cytoplasmic domain
The recombinant form of Epigen is typically produced in E. coli and is available in both carrier-free and carrier-containing formulations. The carrier protein, often Bovine Serum Albumin (BSA), enhances protein stability and shelf-life .
Epigen is known for its ability to induce cell proliferation. It binds to EGF receptors, albeit with lower affinity compared to EGF itself. Despite this lower affinity, Epigen has been shown to be superior in inducing cell proliferation . This makes it a valuable tool in research and therapeutic applications, particularly in studies involving epithelial cell growth and differentiation.
