EPGN Human, Sf9

Epigen is originally synthesized as a glycosylated transmembrane precursor protein with a molecular mass of approximately 14.7 kDa. This precursor undergoes proteolytic cleavage to produce a mature, soluble sequence . The recombinant form of Epigen produced in Sf9 insect cells is a single, glycosylated polypeptide chain containing 97 amino acids and has a molecular mass of 10.8 kDa . It is expressed with a 6-amino acid His tag at the C-terminus and is purified using proprietary chromatographic techniques .

Epigen is a potent mitogen for fibroblasts and epithelial cells . It is produced in various tissues, including the testis, liver, heart, and certain tumor cells . The biological activity of Epigen is determined by its ability to stimulate the proliferation of cells, such as murine Balb/3T3 cells, in a dose-dependent manner .

Epigen (Human Recombinant, Sf9) is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical . The protein is supplied as a sterile filtered colorless solution, formulated in phosphate-buffered saline (PBS) with 10% glycerol . For long-term storage, it is recommended to add a carrier protein such as human serum albumin (HSA) or bovine serum albumin (BSA) to prevent multiple freeze-thaw cycles .

Epigen should be stored at 4°C if it will be used within 2-4 weeks. For longer storage periods, it should be frozen at -20°C . Lyophilized Epigen, although stable at room temperature for up to three weeks, should be stored desiccated below -18°C for extended periods .